Human CD20 F(ab')2 (Research Grade Ibritumomab Biosimilar) Antibody

Catalog #: MAB11054-FAB2 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11054-FAB2-100
MAB11054-FAB2-SP

Detection of CD20 (Ibritumomab) in Human PBMCs by Flow Cytometry.

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Human CD20 F(ab')2 (Research Grade Ibritumomab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD20 in direct ELISAs.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu10F2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human MS4A1/CD20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD20 (Ibritumomab) in Human PBMCs by Flow Cytometry. Human PBMCs were stained with (A) Human Anti-Human CD20 (Research Grade Ibritumomab Biosimilar) Monoclonal Antibody (Catalog # MAB11054-FAB2) or (B) unstained, followed by followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7