Human CD200 PE-conjugated Antibody Summary
Gln31-Gly232
Accession # P41217.3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD200 in Th17 Differentiated Human PBMCs by Flow Cytometry. Th17 differentiated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD200 PE-conjugated Monoclonal Antibody (Catalog # FAB27241P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD200 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD200 PE-conjugated Monoclonal Antibody (Catalog # FAB27241P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: CD200
CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5‑7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a co‑stimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14‑16).
- Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
- Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
- McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
- Wright, G.J. et al. (2001) Immunology 102:173.
- Shiratori, I. et al. (2005) J. Immunol. 175:4441.
- Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
- Fallarino, F. et al. (2004) J. Immunol. 173:3748.
- Hoek, R.M. et al. (2000) Science 290:1768.
- Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
- Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
- Borriello, F. et al. (1997) J. Immunol. 158:4548.
- Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
- Hatherley, D. et al. (2005) J. Immunol. 175:2469.
- Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
- Cameron, C.M. et al. (2005) J. Virol. 79:6052.
- Langlais, C.L. et al. (2006) J. Virol. 80:3098.
Product Datasheets
Citations for Human CD200 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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CD11b and CD200 on Circulating Monocytes Differentiate Two Angiographic Subtypes of Polypoidal Choroidal Vasculopathy
Authors: Y Subhi, M Krogh Niel, CR Molbech, A Oishi, A Singh, MH Nissen, TL Sørensen
Invest. Ophthalmol. Vis. Sci., 2017-10-01;58(12):5242-5250.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
PLoS ONE, 2012-08-14;7(8):e43255.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.
Authors: Ramsay, Alan G, Clear, Andrew J, Fatah, Rewas, Gribben, John G
Blood, 2012-04-30;120(7):1412-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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