Human CD23/Fc epsilon RII Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 158 | 283 | 576 | 151 | 274 | 533 |
Standard Deviation | 13.5 | 17.8 | 40.8 | 13.7 | 22.4 | 40.2 |
CV% | 8.5 | 6.3 | 7.1 | 9.1 | 8.2 | 7.5 |
Recovery
The recovery of CD23 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 105 | 94-114 |
EDTA Plasma (n=4) | 99 | 89-105 |
Heparin Plasma (n=4) | 98 | 90-112 |
Serum (n=4) | 94 | 85-108 |
Urine (n=4) | 100 | 85-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CD23/Fc epsilon RII
CD23, also known as Fc epsilon RII, is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citation for Human CD23/Fc epsilon RII Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Mediating effect of soluble B-cell activation immune markers on the association between anthropometric and lifestyle factors and lymphoma development
Authors: F Saberi Hos, PM Kolijn, D Casabonne, A Nieters, M Solans, S Naudin, P Ferrari, JD Mckay, E Weiderpass, V Perduca, C Besson, FR Mancini, G Masala, V Krogh, F Ricceri, JM Huerta, D Petrova, N Sala, A Trichopoul, A Karakatsan, C La Vecchia, R Kaaks, F Canzian, D Aune, H Boeing, MB Schulze, A Perez-Corn, AW Langerak, VHJ van der Ve, R Vermeulen
Sci Rep, 2020-08-14;10(1):13814.
Species: Human
Sample Types: Serum
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