Human CD25/IL-2R alpha Antibody

Name: Human CD25/IL-2R alpha Antibody
Brand: R&D Systems
SKU: MAB223
Rating: 3 (1 reviews)

Catalog #: MAB223
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB223-SP
MAB223-100
MAB223-500

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All CD25/IL-2R alpha Antibodies

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Citations (4)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed.

Source

Monoclonal Mouse IgG₁ Clone # 22722

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from ascites

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of human lymph node

Immunocytochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CD25/IL‑2 R alpha in Human PBMCs. CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223). The ND₅₀ is typically 0.5-1.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
Nature Communications, 2022-06-03;13(1):3121.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells
Authors: F Porichis, MG Hart, A Massa, HL Everett, A Morou, J Richard, N Brassard, M Veillette, M Hassan, NL Ly, JP Routy, GJ Freeman, M Dubé, A Finzi, DE Kaufmann
J. Immunol., 2018-06-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
Sci Rep, 2016-09-02;6(0):32320.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
Cytokine modulation of defensive rage behavior in the cat: role of GABAA and interleukin-2 receptors in the medial hypothalamus.
Authors: Bhatt S, Zalcman S, Hassanain M, Siegel A
Neuroscience, 2005-01-01;133(1):17-28.
Species: Feline
Sample Types: In Vivo
Applications: Neutralization