Human CD27 Ligand/TNFSF7 Antibody

Name: Human CD27 Ligand/TNFSF7 Antibody
Brand: R&D Systems
SKU: MAB2738
Rating: 4 (1 reviews)

Catalog #: MAB2738
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2738
MAB2738-SP

Detection of CD27Ligand/TNFSF7 in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 by Flow Cytometry.

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All CD27 Ligand/TNFSF7 Antibodies

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Citations (5)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD27 Ligand/TNFSF7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD27 Ligand/TNFSF7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 301731

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7
Gln45-Pro193
Accession # AAA36175

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD27Ligand/TNFSF7 in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB2738) followed by Goat anti-mouse PE-conjugated secondary antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27 Ligand/TNFSF7

CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells.

Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Alternate Names

CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD27 Ligand/TNFSF7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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IL-15-secreting CAR natural killer cells directed toward the pan-cancer target CD70 eliminate both cancer cells and cancer-associated fibroblasts
Authors: Van den Eynde, A;Gehrcken, L;Verhezen, T;Lau, HW;Hermans, C;Lambrechts, H;Flieswasser, T;Quatannens, D;Roex, G;Zwaenepoel, K;Marcq, E;Joye, P;Cardenas De La Hoz, E;Deben, C;Gasparini, A;Montay-Gruel, P;Le Compte, M;Lion, E;Lardon, F;Van Laere, S;Siozopoulou, V;Campillo-Davo, D;De Waele, J;Pauwels, P;Jacobs, J;Smits, E;Van Audenaerde, JRM;
Journal of hematology & oncology
Species: Human hepegivirus
Sample Types: Whole Tissue
Applications: Immunohistochemistry
CD70 is Consistently Expressed in Primary and Metastatic Clear Cell Renal Cell Carcinoma
Authors: Huang, RR;Chen, Z;Kroeger, N;Pantuck, A;Said, J;Kluger, HM;Shuch, B;Ye, H;
Clinical genitourinary cancer
Species: Human hepegivirus
Sample Types: Whole Tissue
CD70 antibody-drug conjugate as a potential therapeutic agent for uterine leiomyosarcoma
Authors: R Nakae, S Matsuzaki, S Serada, K Matsuo, M Shiomi, K Sato, Y Nagase, S Matsuzaki, S Nakagawa, K Hiramatsu, A Okazawa, T Kimura, T Egawa-Taka, E Kobayashi, Y Ueda, K Yoshino, T Naka, T Kimura
Am. J. Obstet. Gynecol., 2020-08-19;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Screening a Broad Range of Solid and Haematological Tumour Types for CD70 Expression Using a Uniform IHC Methodology as Potential Patient Stratification Method
Authors: T Flieswasse, V Camara-Cla, A Danu, J Bosq, V Ribrag, P Zabrocki, L Van Rompae, H de Haard, K Zwaenepoel, E Smits, P Pauwels, J Jacobs
Cancers (Basel), 2019-10-22;11(10):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Targeted alpha therapy using a novel CD70 targeted thorium-227 conjugate in in vitro and in vivo models of renal cell carcinoma
Authors: UB Hagemann, D Mihaylova, SR Uran, J Borrebaek, D Grant, RM Bjerke, J Karlsson, AS Cuthbertso
Oncotarget, 2017-01-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC