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Human CD31/PECAM-1 Antibody

Catalog #: BBA7
36 Citations 7 Reviews Datasheet / COA / SDS
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BBA7
Detection of Human CD31/PECAM‑1 by Western Blot.
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Citations (36)
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Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD31/PECAM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.
Source
Monoclonal Mouse IgG1 Clone # 9G11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Artery in Human Liver
Immunoprecipitation
Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD31/PECAM-1 antibody by Western Blot. View Larger

Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CD31/PECAM‑1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

CD31/PECAM‑1 in HUVEC Human Cells. CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated View Larger

CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

Detection of CD31/PECAM‑1 in Artery in Human Liver. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Artery in Human Liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Human CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Selective ROCK Inhibitor Enhances Blood Flow Recovery after Hindlimb Ischemia
    Authors: Fayed, HS;Bakleh, MZ;Ashraf, JV;Howarth, A;Ebner, D;Al Haj Zen, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry/ Immunofluorescence
  2. PARG suppresses tumorigenesis and downregulates genes controlling angiogenesis, inflammatory response, and immune cell recruitment
    Authors: S Johnson, Y Karpova, D Guo, A Ghatak, DA Markov, AV Tulin
    BMC Cancer, 2022-05-18;22(1):557.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. A Novel High Content Angiogenesis Assay Reveals That Lacidipine, L-Type Calcium Channel Blocker, Induces In Vitro Vascular Lumen Expansion
    Authors: DA Nawrot, LY Ozer, A Al Haj Zen
    International Journal of Molecular Sciences, 2022-04-28;23(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Purification and characterization of human adipose-resident microvascular endothelial progenitor cells
    Authors: N Saito, T Shirado, H Funabashi-, Y Wu, M Mori, R Asahi, K Yoshimura
    Scientific Reports, 2022-02-02;12(1):1775.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
    Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
    Aging Cell, 2021-10-23;0(0):e13501.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Functional Assay, IF
  6. Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy
    Authors: RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker
    International Journal of Molecular Sciences, 2021-10-16;22(20):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
    Species: Human, Rhesus Macaque
    Sample Types: Whole Tissue
    Applications: IHC
  8. Sphingosine 1 Phosphate (S1P) Receptor 1 Is Decreased in Human Lung Microvascular Endothelial Cells of Smokers and Mediates S1P Effect on Autophagy
    Authors: K Goel, EL Beatman, N Egersdorf, A Scruggs, D Cao, EV Berdyshev, KS Schweitzer, I Petrache
    Cells, 2021-05-14;10(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Isolation and characterization of novel primary cells from the human distal outflow pathway
    Authors: U Roy Chowdh, CK Bahler, CR Hann, BH Holman, MP Fautsch
    Scientific Reports, 2021-02-17;11(1):4034.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow
    Authors: RA Santos, KD Asensi, JHO de Barros, RCS de Menezes, IR Cordeiro, JMB Neto, TH Kasai-Brun, RCDS Goldenberg
    International Journal of Molecular Sciences, 2020-12-15;21(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
    Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
    Cell Reports, 2020-12-15;33(11):108514.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  12. Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis
    Authors: C Manferdini, Y Saleh, P Dolzani, E Gabusi, D Trucco, G Filardo, G Lisignoli
    Biology, 2020-12-10;9(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
    Authors: N Mori, Y Akagi, Y Imai, Y Takayama, YS Kida
    Sci Rep, 2020-04-14;10(1):5646.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Extracorporeal Shock Waves Therapy Delivered by Aries Improves Erectile Dysfunction in Spontaneously Hypertensive Rats Through Penile Tissue Remodeling and Neovascularization
    Authors: R Assaly, F Giuliano, P Clement, M Laurin, M Favier, P Teo, J Bernabe, L Alexandre, D Behr-Rouss
    Sex Med, 2019-09-21;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Nintedanib ameliorates experimental pulmonary arterial hypertension via inhibition of endothelial mesenchymal transition and smooth muscle cell proliferation
    Authors: T Tsutsumi, T Nagaoka, T Yoshida, L Wang, S Kuriyama, Y Suzuki, Y Nagata, N Harada, Y Kodama, F Takahashi, Y Morio, K Takahashi
    PLoS ONE, 2019-07-24;14(7):e0214697.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain
    Authors: J Luzuriaga, O Pastor-Alo, JM Encinas, F Unda, G Ibarretxe, JR Pineda
    Front Physiol, 2019-03-28;10(0):347.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Straightforward method for singularized and region-specific CNS microvessels isolation
    Authors: J Rose Dayto, MC Franke, Y Yuan, L Cruz-Oreng
    J. Neurosci. Methods, 2019-02-21;0(0):.
    Species: Mouse, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  18. Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
    Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
    PLoS ONE, 2018-10-09;13(10):e0204177.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  19. Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability
    Authors: S Li, LR Nih, H Bachman, P Fei, Y Li, E Nam, R Dimatteo, ST Carmichael, TH Barker, T Segura
    Nat Mater, 2017-08-07;16(9):953-961.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
    Stem Cell Reports, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms
    Authors: Lopa Leach
    Placenta, 2016-10-21;48(0):87-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling
    Stem Cell Res Ther, 2016-08-17;7(1):115.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  23. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
    Sci Rep, 2016-07-18;6(0):29884.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Endothelial Surface Protrusion by a Point Force
    Authors: Y Chen, L Lu, JY Shao
    Biophys. J, 2016-03-08;110(5):1150-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.
    Authors: Weston, Chris J, Shepherd, Emma L, Claridge, Lee C, Rantakari, Pia, Curbishley, Stuart M, Tomlinson, Jeremy W, Hubscher, Stefan G, Reynolds, Gary M, Aalto, Kristiin, Anstee, Quentin, Jalkanen, Sirpa, Salmi, Marko, Smith, David J, Day, Christop, Adams, David H
    J Clin Invest, 2014-12-22;125(2):501-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
    Authors: Martinelli R, Zeiger A, Whitfield M, Sciuto T, Dvorak A, Van Vliet K, Greenwood J, Carman C
    J Cell Sci, 2014-07-07;127(0):3720-34.
    Species: Human, Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  27. Association between PON1 rs662 polymorphism and coronary artery disease.
    Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
    Eur J Clin Nutr, 2014-06-11;68(9):1029-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
    Authors: Kanasaki K, Shi S, Kanasaki M, He J, Nagai T, Nakamura Y, Ishigaki Y, Kitada M, Srivastava S, Koya D
    Diabetes, 2014-02-26;63(6):2120-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. The role of endothelial cell attachment to elastic fibre molecules in the enhancement of monolayer formation and retention, and the inhibition of smooth muscle cell recruitment.
    Authors: Williamson MR, Shuttleworth A, Canfield AE, Black RA, Kielty CM
    Biomaterials, 2007-09-11;28(35):5307-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007-02-08;109(10):4264-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Mechanisms of endothelial cell protection by blockade of the JAK2 pathway.
    Authors: Neria F, Caramelo C, Peinado H, Gonzalez-Pacheco FR, Deudero JJ, de Solis AJ, Fernandez-Sanchez R, Penate S, Cano A, Castilla MA
    Am. J. Physiol., Cell Physiol., 2006-10-11;292(3):C1123-31.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  32. Disruption of graft endothelium correlates with early failure after off-pump coronary artery bypass surgery.
    Authors: Manchio JV, Gu J, Romar L, Brown J, Gammie J, Pierson RN, Griffith B, Poston RS
    Ann. Thorac. Surg., 2005-06-01;79(6):1991-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  33. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004-10-25;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.
    Authors: Wei H, Song J, Fang L, Li G, Chatterjee S
    Biochem. Biophys. Res. Commun., 2004-08-06;320(4):1228-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  35. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004-03-01;15(3):566-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Stimulation of primary human endothelial cell proliferation by IFN.
    Authors: Gomez D, Reich NC
    J. Immunol., 2003-06-01;170(11):5373-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CD31/PECAM-1 Antibody

Average Rating: 4.1 (Based on 7 Reviews)

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Human CD31/PECAM-1 Antibody
By Anonymous on 03/09/2022
Application: WB Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Western Blot Human CD31/PECAM-1 Antibody BBA7
Human CD31/PECAM-1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human CD31/PECAM-1 Antibody BBA7
Human CD31/PECAM-1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human CD31/PECAM-1 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human CD31/PECAM-1 Antibody
By Anonymous on 02/28/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Antibody was reconstituted at 0.5mg/ml and used at 1:50 dilution overnight in 4ºC.

Immunocytochemistry/Immunofluorescence Human CD31/PECAM-1 Antibody BBA7
Human CD31/PECAM-1 Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: artery Species: Human

Frozen human artery. Permeabilized with 80% acetone.
Primary antibody at 1:100 incubate at 4oC overnight
Secondary antibody: CY3 donkey anti-mouse secondary antibody. 1 hour at room temperature.

Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7
Human CD31/PECAM-1 Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: Muscle tissue Species: Human

Antigen retrieval: 10mM Citrate pH6.
Primary antibody at 1:100 incubate at 4oC overnight
Secondary antibody: Alexa 647 donkey anti-mouse secondary antibody. 1 hour at room temperature.

Immunohistochemistry Human CD31/PECAM-1 Antibody BBA7