Human CD34 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 APC-conjugated Monoclonal Antibody (Catalog # FAB7227A) and Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 APC-conjugated Monoclonal Antibody (Catalog # FAB7227A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD34
The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
Product Datasheets
Citations for Human CD34 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Structure-guided engineering of immunotherapies targeting TRBC1 and TRBC2 in T cell malignancies
Authors: Ferrari, M;Righi, M;Baldan, V;Wawrzyniecka, P;Bulek, A;Kinna, A;Ma, B;Bughda, R;Akbar, Z;Srivastava, S;Gannon, I;Robson, M;Sillibourne, J;Jha, R;El-Kholy, M;Amin, OM;Kokalaki, E;Banani, MA;Hussain, R;Day, W;Lim, WC;Ghongane, P;Hopkins, JR;Jungherz, D;Herling, M;Welin, M;Surade, S;Dyson, M;McCafferty, J;Logan, D;Cordoba, S;Thomas, S;Sewell, A;Maciocia, P;Onuoha, S;Pule, M;
Nature communications
Species: Human hepegivirus, Murine polyomavirus strain A3
Sample Types: Whole Cells
Applications: Flow Cytometry, Immunocytochemistry -
Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients
Authors: A Sen, A Singh, A Roy, S Mohanty, N Naik, M Kalaivani, L Ramakrishn
Scientific Reports, 2022-02-23;12(1):3076.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
BMC Oral Health, 2021-09-24;21(1):470.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeted multi-epitope switching enables straightforward positive/negative selection of CAR T cells
Authors: L Mosti, LM Langner, KO Chmielewsk, P Arbuthnot, J Alzubi, T Cathomen
Gene Therapy, 2021-02-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification and Targeting of Thomsen-Friedenreich and IL1RAP Antigens on Chronic Myeloid Leukemia Stem Cells Using Bi-Specific Antibodies
Authors: RE Eldesouki, C Wu, FM Saleh, EA Mohammed, S Younes, NE Hassan, TC Brown, EU Alt, JE Robinson, FM Badr, SE Braun
OncoTargets and Therapy, 2021-01-22;14(0):609-621.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T�cell functionality
Authors: M Sachdeva, BW Busser, S Temburni, B Jahangiri, AS Gautron, A Maréchal, A Juillerat, A Williams, S Depil, P Duchateau, L Poirot, J Valton
Nat Commun, 2019-11-13;10(1):5100.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Constitutively active MyD88/CD40 costimulation enhances expansion and efficacy of chimeric antigen receptor T cells targeting hematological malignancies
Authors: MR Collinson-, WC Chang, A Lu, M Khalil, JW Crisostomo, PY Lin, A Mahendrava, NP Shinners, ME Brandt, M Zhang, M Duong, JH Bayle, KM Slawin, DM Spencer, AE Foster
Leukemia, 2019-02-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: CAR-T (Flow Cytometry) -
A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies
Authors: J Valton, V Guyot, B Boldajipou, C Sommer, T Pertel, A Juillerat, A Duclert, BJ Sasu, P Duchateau, L Poirot
Sci Rep, 2018-06-12;8(1):8972.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chimeric Antigen Receptor-Engineered Human Gamma Delta T Cells: Enhanced Cytotoxicity with Retention of Cross Presentation
Authors: A Capsomidis, G Benthall, HH Van Acker, J Fisher, AM Kramer, Z Abeln, Y Majani, T Gileadi, R Wallace, K Gustafsson, B Flutter, J Anderson
Mol. Ther., 2017-12-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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