Human CD39/ENTPD1 Antibody

Catalog #: MAB43971 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43971
MAB43971-SP

All CD39/ENTPD1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD39/ENTPD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD39 or recombinant human CD39L2, 3, or 4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 498409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD39
Thr38-Val478
Accession # P49961

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD39/ENTPD1 (Catalog # 4397-EN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), also called NTPDase-1 and designated CD39, is an integral membrane protein with an extracellular active site. ENTPD1 was originally described as a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). ENTPD1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling (4). ENTPD1 is involved in the processes of thromboregulation and vascular inflammation (5). The administration of soluble ENTPD1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6). The amino acid sequence of human ENTPD1 shares 77% and 75% identity with that of mouse and rat ENTPD1, respectively.

References

Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
Rowe, M. et al. (1982) Int. J. Cancer 29:373.
Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Entrez Gene IDs

953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)

Alternate Names

ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1