Human CD4 Antibody

Name: Human CD4 Antibody
Brand: R&D Systems
SKU: AF-379-NA
Rating: 3.5 (2 reviews)

Catalog #: AF-379-NA
13 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-379-NA
AF-379-SP

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All CD4 Antibodies

Product Details

Citations (13)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Lys26-Trp390
Accession # P01730

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells

Immunohistochemistry

1-15 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil tissue

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CD4 in Human T Cells. CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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CD4 in Human Tonsil Tissue. CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Western Blot

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Detection of Human CD4 by Western Blot. Western blot shows lysates of PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD4 at approximately ~55kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human CD4 by Simple Western^TM. Simple Western lane view shows lysates of Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
Authors: R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan
Journal of personalized medicine, 2022-04-13;12(4):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses
Authors: K Sangare, S Helmold Ha, M Moore, C Hogge, T Hoang, MA Rahman, DJ Venzon, C LaBranche, D Montefiori, M Robert-Gur, MA Thomas
Vaccines, 2022-02-15;10(2):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cyomtetry, Western Blot
Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
Biology, 2021-09-18;10(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia
Authors: JP Sutherland, M Steain, ME Buckland, M Rodriguez, AL Cunningham, B Slobedman, A Abendroth
Front Microbiol, 2019-09-11;10(0):2117.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
J Clin Invest, 2014-12-22;125(2):739-51.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells.
Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S
J Virol, 2014-07-09;88(19):11045-55.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo.
Authors: Suzuki, Yasuhiro, Gatanaga, Hiroyuki, Tachikawa, Natsuo, Oka, Shinichi
PLoS ONE, 2014-02-06;9(2):e86479.
Species: Human
Sample Types: Whole Cells
Applications: ICC
A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
Authors: Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J
PLoS Pathog, 2012-09-20;8(9):e1002937.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells.
Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V
J. Immunol., 2009-09-14;183(7):4792-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies.
Authors: Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE
Arthritis Rheum., 2007-01-01;56(1):372-83.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
Authors: Wang B, He J, Liu C, Chang LJ
Vaccine, 2006-02-28;24(17):3477-89.
Species: Human, Primate
Sample Types: Whole Cells
Applications: ICC

Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress.
Authors: Bjerke SL, Roller RJ
Virology, 2006-01-19;347(2):261-76.
Species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICC