Human CD68/SR-D1 Antibody

Catalog #: MAB2040
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2040
MAB2040-SP

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All CD68/SR-D1 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD68/SR-D1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD68/SR‑D1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 298813

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Dual RNAscope ISH-IHC

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CD68/SR‑D1 in Human Tonsil. CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB2040) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible

CD68/SR-D1 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC.

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CD68/SR-D1 in Human Tonsil Using Dual RNAscope^®ISH and IHC. CD68/SR-D1 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope^®Probe (Catalog # 560591) followed by immunohistochemistry using R&D Systems Mouse Anti-Human CD68/SR-D1 Monoclonal Antibody (Catalog# MAB2040) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (Catalog # 322500). Specific staining was localized to cytoplasm in lymphocytes.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Alternate Names

CD_antigen: CD68; CD68 antigen; CD68 molecule; CD68; DKFZp686M18236; gp110; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD68/SR-D1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
Histopathology, 2005-06-01;46(6):659-67.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P