Human CD81 Antibody

Catalog #: MAB46152 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB46152-100
MAB46152-SP

Detection of Human CD81 by Western Blot.

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All CD81 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD81 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD81 in Elisas.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2664C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

CHO-derived human CD81 protein
Phe113-Ala201
Accession # P60033

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

4 µg/mL

Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line

Simple Western

10 µg/mL

SK-Mel-28 human malignant melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD81 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD81 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human CD81 by Simple Western^TM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD81 at approximately 27 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD81

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).

References

Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
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Zhu, Y.-Z. et al. (2012) Virology 429:112.
Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
Takahashi, S. et al. (1990) J. Immunol. 145:2207.
Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
Liu, B. et al. (2009) Am. J. Pathol. 175:717.
Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
Boismenu, R. et al. (1996) Science 271:198.
Silvie, O. et al. (2003) Nat. Med. 9:93.
Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
Pileri, P. et al. (1998) Science 282:938.
Crotta, S. et al. (2002) J. Exp. Med. 195:35.
Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
Tu, Z. et al. (2013) Cell. Immunol. 284:98.

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Alternate Names

CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7