Human CD8 alpha Antibody

Name: Human CD8 alpha Antibody
Brand: R&D Systems
SKU: MAB3801
Rating: 4 (2 reviews)

Catalog #: MAB3801
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3801-100
MAB3801-SP

1 Image

All CD8 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD8 alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD8 alpha in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 37020

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741696

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

CD8 alpha in Human Tonsil. CD8a was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB3801) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD8 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma
Authors: C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch
Journal of personalized medicine, 2021-02-10;11(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC