Human CD94 APC-conjugated Antibody

Catalog #: FAB1058A Datasheet / COA / SDS

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FAB1058A

Detection of CD94 in Human PBMCs by Flow Cytometry.

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Human CD94 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.

Source

Monoclonal Mouse IgG₁ Clone # 131412

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD94 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human CD94 APC-conjugated Monoclonal Antibody (Catalog # FAB1058A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD94

CD94 is a 30 kDa type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer (NK) cells and a subset of CD8⁺ T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with a NKG2 family member. The CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, the CD94/NKG2C heterodimer associates with the DAP12 adaptor protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins.

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Alternate Names

CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor