Human CD96 v2 APC-conjugated Antibody

Catalog #: FAB6199A
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FAB6199A

Detection of CD96v2 in Human PBMC Lymphocytes by Flow Cytometry.

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All CD96 Antibodies

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Human CD96 v2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD96 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 628211

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD96
Val22-Asp501
Accession # NP_005807

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD96v2 in Human PBMC Lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human CD96v2 APC-conjugated Monoclonal Antibody (Catalog # FAB6199A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD96

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4⁺ and CD8⁺ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155⁺ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile

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Citation for Human CD96 v2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1