Human CD98 APC-conjugated Antibody

Catalog #: FAB5920A Datasheet / COA / SDS

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FAB5920A

Detection of CD98 in K562 Human Cell Line by Flow Cytometry.

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Human CD98 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD98 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 590559

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD98
Asp105-Gln529
Accession # P08195

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD98 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human CD98 APC-conjugated Monoclonal Antibody (Catalog # FAB5920A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD98

CD98, also known as 4F2hc, is a ubiquitously expressed, 85-95 kDa member of the SLC3 (solute carrier 3) family of amino acid transporters. It is a type II transmembrane glycoprotein that covalently-associates with a variable number of small, 38-45 kDa, 12-transmembrane proteins that belong to the SLC7 family of molecules. The 120-130 kDa heterdimeric 4F2 complex is known to transport neutral or cationic amino acids with, or without, a contribution of sodium. It also interacts with Integrin beta 1 and beta 3 to promote cell polarization and migration. Notably, CD98 is "vertebrate-restricted," and its appearance evolutionarily has been linked to the onset of tumorigenesis. Over amino acids (aa) 105-529, human and mouse CD98 share 76% aa sequence identity.

Entrez Gene IDs

6520 (Human); 17254 (Mouse)

Alternate Names

4F2; 4F2hc; 4T2HC; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC; CD98 antigen; CD98 heavy chain; CD98; CD98HC; CH98hc; FRP-1; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1antigen defined by monoclonal 4F2, heavy chain; monoclonal 44D7; NACAE4F2 cell-surface antigen heavy chain; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen