Human CEACAM-3/CD66d Antibody

Catalog #: MAB4166 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4166
MAB4166-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEACAM-3/CD66d Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM‑3/CD66d in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -4, -5, or -6 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 417009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
Lys35-Gly155
Accession # P40198

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CEACAM‑3/CD66d

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-3/CD66d

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3, (CEACAM-3) also known as CD66d and CGM1, is a 35 kDa transmembrane glycoprotein in the CEACAM family of the immunoglobulin superfamily. The extracellular region consists of one Ig-like V-type domain, and the cytoplasmic tail contains one ITAM. CEACAM-3 is upregulated on activated neutrophils and mediates their adhesion to vascular endothelial cells. CEACAM-3 binds the Opa opacity proteins on some Gram negative bacteria, leading to bacterial uptake and neutrophil death. Alternate splicing generates an isoform with a substituted cytoplasmic tail and a potentially secreted isoform that contains a highly charged and Ser/Thr-rich sequence in place of the transmembrane and cytoplasmic regions. Orthologs of human CEACAM-3 have not been identified in other species.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3

Entrez Gene IDs

1084 (Human); 384557 (Mouse); 24926 (Rat)

Alternate Names

CD66d; CEA; CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3; CEACAM-3; CGM1; W264; W282