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Human CEACAM-6/CD66c Antibody

Catalog #: MAB3934 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3934
MAB3934-SP
Detection of CEACAM‑6/CD66c in Human Granulocytes by Flow Cytometry.
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Human CEACAM-6/CD66c Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed.
Source
Monoclonal Mouse IgG2A Clone # 439424
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c
Lys35-Gly320
Accession # P40199
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CEACAM‑6/CD66c (Catalog # 3934-CM)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CEACAM‑6/CD66c antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of CEACAM‑6/CD66c in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Mouse Anti-Human CEACAM-6/CD66c Monoclonal Antibody (Catalog # MAB3934, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of CEACAM‑6/CD66c antibody in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP antibody by Flow Cytometry. View Larger

Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-6/CD66c Monoclonal Antibody (Catalog # MAB3934) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific cross‑reacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
  3. Barnett, T. et al. (1988) Genomics 3:59.
  4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
  5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
  6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
  7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
  9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
  10. Kalina, T. et al. (2005) BMC Cancer 5:38.
  11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
  14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
Entrez Gene IDs
4680 (Human); 107126399 (Cynomolgus Monkey)
Alternate Names
carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen); carcinoembryonic antigen-related cell adhesion molecule 6; CD66c antigen; CD66c; CEACAM6; CEACAM-6; CEAL; NCA; NCACEAL; Non-specific crossreacting antigen; Normal cross-reacting antigen

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Human CEACAM-6/CD66c Antibody
By Anonymous on 08/09/2021
Application: WB Sample Tested: HT-29 human colon adenocarcinoma cell line Species: Human
Western Blot Human CEACAM-6/CD66c Antibody MAB3934