Human CFTR C-Terminus Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: CFTR
Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).
Product Datasheets
Citations for Human CFTR C-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancer
Authors: SM Snow, KA Matkowskyj, M Maresh, L Clipson, TN Vo, KA Johnson, DA Deming, MA Newton, WM Grady, PJ Pickhardt, RB Halberg
Human pathology, 2021-10-14;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SFPQ rescues F508del-CFTR expression and function in cystic fibrosis bronchial epithelial cells
Authors: P Kumar, DK Soni, C Sen, MB Larsen, K Mazan-Mamc, Y Piao, S De, M Gorospe, RA Frizzell, R Biswas
Scientific Reports, 2021-08-17;11(1):16645.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Vav3 Mediates Pseudomonas aeruginosa Adhesion to the Cystic Fibrosis Airway Epithelium
Authors: M Badaoui, A Zoso, T Idris, M Bacchetta, J Simonin, S Lemeille, B Wehrle-Hal, M Chanson
Cell Rep, 2020-07-07;32(1):107842.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation
Authors: G Ortiz-Muno, MA Yu, E Lefrançais, B Mallavia, C Valet, JJ Tian, S Ranucci, KM Wang, Z Liu, N Kwaan, D Dawson, ME Kleinhenz, FT Khasawneh, PM Haggie, AS Verkman, MR Looney
J. Clin. Invest., 2020-04-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Treatment of Cystic Fibrosis Patients Homozygous for F508del with Lumacaftor-Ivacaftor (Orkambi�) Restores Defective CFTR Channel Function in Circulating Mononuclear Cells
Authors: M Favia, C Gallo, L Guerra, D De Venuto, A Diana, AM Polizzi, P Montemurro, MA Mariggiò, G Leonetti, A Manca, V Casavola, M Conese
Int J Mol Sci, 2020-03-31;21(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cyclic compression increases F508 Del CFTR expression in ciliated human airway epithelium
Authors: Marozkina N, Bosch J, Cotton C et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
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Clinically-approved CFTR modulators rescue Nrf2 dysfunction in cystic fibrosis airway epithelia
Authors: DC Borcherdin, ME Siefert, S Lin, J Brewington, H Sadek, JP Clancy, SM Plafker, AG Ziady
J. Clin. Invest., 2019-05-30;130(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation -
Effect of dendritic organ ligation on striped eel catfish Plotosus lineatus osmoregulation
Authors: S Malakpour, J Coimbra, JM Wilson
PLoS ONE, 2018-10-23;13(10):e0206206.
Species: Fish - Plotosus lineatus (Striped Eel Catfish)
Sample Types: Whole Tissue
Applications: IHC-P -
Assembly and Functional Analysis of an S/MAR Based Episome with the Cystic Fibrosis Transmembrane Conductance Regulator Gene
Authors: D De Rocco, B Pompili, S Castellani, E Morini, L Cavinato, G Cimino, MA Mariggiò, S Guarnieri, M Conese, P Del Porto, F Ascenzioni
Int J Mol Sci, 2018-04-17;19(4):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling
Authors: A Schögler, F Blank, M Brügger, S Beyeler, SA Tschanz, N Regamey, C Casaulta, T Geiser, MP Alves
Respir. Res., 2017-12-28;18(1):215.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages
Authors: CL Shrestha, KD Assani, H Rinehardt, F Albastroiu, S Zhang, R Shell, AO Amer, LS Schlesinge, BT Kopp
PLoS ONE, 2017-10-05;12(10):e0186169.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5, and CFTR
Authors: MF Figueira, RC Castiglion, CM de Lemos B, FM Ornellas, G da Silva F, MM Morales, RN da Fonseca, J de Souza-M
Physiol Rep, 2017-07-01;5(13):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ammonia exposure affects the mRNA and protein expression levels of certain Rhesus glycoproteins in the gills of climbing perch
Authors: XL Chen, B Zhang, YR Chng, JLY Ong, SF Chew, WP Wong, SH Lam, T Nakada, YK Ip
J. Exp. Biol., 2017-06-02;0(0):.
Species: Fish
Sample Types: Whole Tissue
Applications: IHC-P -
Synergy of cAMP and calcium signaling pathways in CFTR regulation
Authors: Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay
Proc. Natl. Acad. Sci. U.S.A, 2017-02-27;0(0):.
Species: Human
Sample Types: Whole Cells
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Deep interactome profiling of membrane proteins by co-interacting protein identification technology
Nat Protoc, 2016-11-17;11(12):2515-2528.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
New use for an old drug: COX-independent anti-inflammatory effects of sulindac in models of cystic fibrosis
Authors: Agathe Tarze
Br. J. Pharmacol., 2016-04-21;173(11):1728-41.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Western Blot -
CFTR and sphingolipids mediate hypoxic pulmonary vasoconstriction.
Authors: Tabeling C, Yu H, Wang L, Ranke H, Goldenberg N, Zabini D, Noe E, Krauszman A, Gutbier B, Yin J, Schaefer M, Arenz C, Hocke A, Suttorp N, Proia R, Witzenrath M, Kuebler W
Proc Natl Acad Sci U S A, 2015-03-17;112(13):E1614-23.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells.
Authors: Ferru-Clement R, Fresquet F, Norez C, Metaye T, Becq F, Kitzis A, Thoreau V
PLoS ONE, 2015-03-13;10(3):e0118943.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of DeltaF508-cystic fibrosis transmembrane conductance regulator (DeltaF508-CFTR).
Authors: Caohuy H, Yang Q, Eudy Y, Ha T, Xu A, Glover M, Frizzell R, Jozwik C, Pollard H
J Biol Chem, 2014-11-10;289(52):35953-68.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot -
Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels.
Authors: Hassan F, Xu X, Nuovo G, Killilea D, Tyrrell J, Da Tan C, Tarran R, Diaz P, Jee J, Knoell D, Boyaka P, Cormet-Boyaka E
Respir Res, 2014-06-23;15(0):69.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells.
Authors: Carbone A, Castellani S, Favia M, Diana A, Paracchini V, Di Gioia S, Seia M, Casavola V, Colombo C, Conese M
J Cell Mol Med, 2014-06-03;18(8):1631-43.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
Authors: Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M
J Immunol, 2014-04-14;192(10):4804-12.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water.
Authors: Li Z, Lui E, Wilson J, Ip Y, Lin Q, Lam T, Lam S
PLoS ONE, 2014-01-31;9(1):e87591.
Species: Fish
Sample Types: Whole Tissue
Applications: IHC-P -
Correcting the cystic fibrosis disease mutant, A455E CFTR.
Authors: Cebotaru L, Rapino D, Cebotaru V, Guggino W
PLoS ONE, 2014-01-08;9(1):e85183.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Western Blot -
RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
J Biol Chem, 2013-09-09;288(43):31177-91.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Phosphorylated C/EBPbeta influences a complex network involving YY1 and USF2 in lung epithelial cells.
Authors: Viart V, Varilh J, Lopez E, Rene C, Claustres M, Taulan-Cadars M
PLoS ONE, 2013-04-01;8(4):e60211.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Regulation of cystic fibrosis transmembrane conductance regulator by microRNA-145, -223, and -494 is altered in DeltaF508 cystic fibrosis airway epithelium.
Authors: Oglesby I, Chotirmall S, McElvaney N, Greene C
J Immunol, 2013-02-22;190(7):3354-62.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.
Authors: Duan, Yuanyuan, Sun, Ying, Zhang, Fan, Zhang, Wei Kevi, Wang, Dong, Wang, Yan, Cao, Xu, Hu, Wenbao, Xie, Changyan, Cuppoletti, John, Magin, Thomas M, Wang, Haixia, Wu, Zhenguo, Li, Ning, Huang, Pingbo
J Biol Chem, 2012-10-08;287(48):40547-59.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.
Authors: Murphy, Sean V, Lim, Rebecca, Heraud, Philip, Cholewa, Marian, Le Gros, Mark, de Jonge, Martin D, Howard, Daryl L, Paterson, David, McDonald, Courtney, Atala, Anthony, Jenkin, Graham, Wallace, Euan M
PLoS ONE, 2012-09-28;7(9):e46533.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
Role of binding and nucleoside diphosphate kinase A in the regulation of the cystic fibrosis transmembrane conductance regulator by AMP-activated protein kinase.
Authors: King, J Darwin, Lee, Jeffrey, Riemen, Claudia, Neumann, Dietbert, Xiong, Sheng, Foskett, J Kevin, Mehta, Anil, Muimo, Richmond, Hallows, Kenneth
J Biol Chem, 2012-08-06;287(40):33389-400.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Proteomic Identification of Calumenin as a G551D - CFTR Associated Protein.
Authors: Teng L, Kerbiriou M, Taiya M, Le Hir S, Mignen O, Benz N, Trouve P, Ferec C
PLoS ONE, 2012-06-29;7(6):e40173.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.
Authors: Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P
PLoS ONE, 2011-07-21;6(7):e22212.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
Authors: Fouassier L, Rosenberg P, Mergey M, Saubamea B, Claperon A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
Am. J. Pathol., 2009-03-01;174(3):869-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Cystic fibrosis transmembrane regulator missing the first four transmembrane segments increases wild type and DeltaF508 processing.
Authors: Cebotaru L, Vij N, Ciobanu I, Wright J, Flotte T, Guggino WB
J. Biol. Chem., 2008-05-28;283(32):21926-33.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in human bone.
Authors: Shead EF, Haworth CS, Condliffe AM, McKeon DJ, Scott MA, Compston JE
Thorax, 2007-07-01;62(7):650-1.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Expression and function of cystic fibrosis transmembrane conductance regulator in rat intrapulmonary arteries.
Authors: Robert R, Savineau JP, Norez C, Becq F, Guibert C
Eur. Respir. J., 2007-06-27;30(5):857-64.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation -
Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones.
Authors: Madsen SS, Jensen LN, Tipsmark CK, Kiilerich P, Borski RJ
J. Endocrinol., 2007-01-01;192(1):249-60.
Species: Fish
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
The ichthyotoxic alga Chattonella marina induces Na+, K+ -ATPase, and CFTR proteins expression in fish gill chloride cells in vivo.
Authors: Tang JY, Au DW
Biochem. Biophys. Res. Commun., 2006-12-04;353(1):98-103.
Species: Fish - Goldline seabream
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
CFTR Expression in human neutrophils and the phagolysosomal chlorination defect in cystic fibrosis.
Authors: Painter RG, Valentine VG, Lanson NA, Leidal K, Zhang Q, Lombard G, Thompson C, Viswanathan A, Nauseef WM, Wang G
Biochemistry, 2006-08-29;45(34):10260-9.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Absence of typical unfolded protein response in primary cultured cystic fibrosis airway epithelial cells.
Authors: Nanua S, Sajjan U, Keshavjee S, Hershenson MB
Biochem. Biophys. Res. Commun., 2006-03-03;343(1):135-43.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
Authors: Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):72-83.
Species: Ovine
Sample Types: Whole Tissue
Applications: IHC-Fr -
Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype.
Authors: Clain J, Lehmann-Che J, Dugueperoux I, Arous N, Girodon E, Legendre M, Goossens M, Edelman A, de Braekeleer M, Teulon J, Fanen P
Hum. Mutat., 2005-04-01;25(4):360-71.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation -
Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity.
Authors: Bilan F, Thoreau V, Nacfer M, Derand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A
J. Cell. Sci., 2004-03-23;117(0):1923-35.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
Authors: Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH
Biochim. Biophys. Acta, 2002-11-13;1566(1):28-43.
Species: Xenopus
Sample Types: Whole Tissue
Applications: IHC-P -
Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models.
Authors: Pan J, Bear C, Farragher S, Cutz E, Yeger H
Am. J. Respir. Cell Mol. Biol., 2002-11-01;27(5):553-60.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.
Authors: Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
J. Biol. Chem., 2002-04-05;277(24):21480-8.
Species: Amphibian
Sample Types: Cell Lysates
Applications: Western Blot
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Also used in Western blots. The dilution factor for the Western blots was 1:50, so it's not the most specific antibody against fish CFTR, however, the banding was clear and the immunohistochemistry came out adequately.