Human ChemR23 PE-conjugated Antibody

Catalog #: FAB362P Datasheet / COA / SDS

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FAB362P

Detection of ChemR23 in Human Blood Monocytes by Flow Cytometry.

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Human ChemR23 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ChemR23. Stains human ChemR23-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₃ Clone # 84939

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human ChemR23 Met1-Leu371 Accession # NP_004063

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ChemR23 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PerCP-conjugated Monoclonal Antibody (FAB301C) and either (A) Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P) or (B) Mouse IgG₃Phycoerythrin Isotype Control (IC007P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of ChemR23 in HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP by Flow Cytometry. HEK293 human cell line (A) transfected with human ChemR23 or (B) irrelevant protein and eGFP, were stained with Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P). Quadrant markers were set based on Mouse IgG3 PE Isotype Control (IC007P, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: ChemR23

ChemR23 is a chemoattractant receptor expressed on dendritic cells and activated macrophages. ChemR23 binds Chemerin and functions as a coreceptor for SIV and some primary HIV-1 strains.

References

Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Entrez Gene IDs

1240 (Human); 14747 (Mouse); 60669 (Rat)

Alternate Names

CHEMERINR; chemokine receptor-like 1; chemokine-like receptor 1; ChemR23; cmklr1; Dez; Gpcr27; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; MGC126105; MGC126106; orphan G-protein coupled receptor, Dez