Human CHRM3 Antibody

Catalog #: MAB6378 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6378
MAB6378-SP

Detection of CHRM3 in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CHRM3 Antibody Summary

Species Reactivity

Human

Specificity

Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA

Source

Monoclonal Mouse IgG_2B Clone # 580011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CHRM3
Accession # P20309

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex) tissue

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CHRM3 in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CHRM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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CHRM3 in Human Brain (Cortex) Tissue. CHRM3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM3

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

Long Name

Cholinergic Receptor Muscarinic 3

Entrez Gene IDs

1131 (Human); 12671 (Mouse); 24260 (Rat)

Alternate Names

cholinergic receptor, muscarinic 3; CHRM3; HM3; m3 muscarinic receptor; mAChR M3; muscarinic acetylcholine receptor M3