Human Chymase/CMA1 Antibody

Catalog #: MAB4099
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4099
MAB4099-SP

Detection of Human Chymase/CMA1 by Western Blot.

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All Chymase/CMA1 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chymase/CMA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chymase/CMA1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) MCPT-1, rmMCPT-6, rmMCPT-7, or rmMCPT-11.

Source

Monoclonal Mouse IgG_2A Clone # 422818

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chymase/CMA1
Gly20-Asn247
Accession # P23946

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Chymase/CMA1 (Catalog # 4099-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Chymase/CMA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA

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Human Chymase/CMA1 ELISA Standard Curve. Recombinant Human Chymase/CMA1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (MAB40991) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Chymase/CMA1 Monoclonal Antibody (Catalog # MAB4099) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chymase/CMA1

Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells (1). They are synthesized as inactive precursors containing a 2‑residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity (2). Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure (3). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines (4). Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References

Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. ed. p. 1531, Academic Press, San Diego.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Miyazaki, M. and S. Takai (2006) J. Pharmacol. Sci. 100:391.
Nakajima, M. and N. Naya (2002) Jpn. J. Pharmacol. 90:206.

Entrez Gene IDs

1215 (Human); 17228 (Mouse); 25627 (Rat)

Alternate Names

Alpha-chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Chymase; chymase, heart; chymase, mast cell; CMA1; CYH; CYM; EC 3.4.21; EC 3.4.21.39; Mast cell protease I; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Chymase/CMA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload
Authors: PC Powell, CC Wei, L Fu, B Pat, WE Bradley, JF Collawn, LJ Dell'Itali
Heliyon, 2019-04-04;5(4):e01397.
Species: Human, Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Chymase mediates angiotensin-(1-12) metabolism in normal human hearts
Authors: Sarfaraz Ahmad, Chih-Chang Wei, Jose Tallaj, Louis J. Dell’Italia, Norihito Moniwa, Jasmina Varagic et al.
Journal of the American Society of Hypertension
Chymase-Dependent Generation of Angiotensin II from Angiotensin-(1-12) in Human Atrial Tissue
Authors: Sarfaraz Ahmad, Tony Simmons, Jasmina Varagic, Norihito Moniwa, Mark C. Chappell, Carlos M. Ferrario
PLoS ONE