Human CLCA1 Antibody

Catalog #: MAB10766 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10766-100
MAB10766-SP

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Human CLCA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects CLCA1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1022517

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived CLCA1
Asn22-Ser477
Accession # A8K714

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Simple Western

20 µg/mL

Human small intestine

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human colon

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CCL1/I‑309/TCA‑3 in Human Colon. CCL1/I‑309/TCA‑3 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB10766) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human CLCA1 by Simple Western^TM. Simple Western lane view shows lysates of human small intestine, loaded at 0.2 mg/mL. Specific bands were detected for CLCA1 at approximately 55 and 89 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CLCA1 Monoclonal Antibody (Catalog # MAB10766). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLCA1

CLCA1 is a member of the calcium sensitive chloride conductance protein family which may be involved in mediating calcium-activated chloride conductance. This protein is expressed in goblet cells and intestinal basal crypt epithelial of the small intestine and colon. CLCA1 regulates mucin expression and participates in innate immune responses by binding to yet unidentified molecules on inflammatory cells for cytokine and chemokine production. CLCA1 also targets lymphatic endothelial cells and cancer cells. CLCA1 is a candidate as a modulating factor in the pathogenesis of mucus hypersecretory-associated respiratory and gastrointestinal diseases including asthma, COPD, cystic fibrosis, pneumonia, colon colitis, cystic fibrosis intestinal mucous disease, ulcerative colitis, and gastrointestinal parasitic infection.

Long Name

Chloride Channel Accessory 1

Entrez Gene IDs

1179 (Human); 23844 (Mouse); 308015 (Rat)

Alternate Names

CaCC; CaCC-1; Calcium-activated chloride channel family member 1; Calcium-activated chloride channel protein 1; chloride channel accessory 1; chloride channel regulator 1; chloride channel regulator; chloride channel, calcium activated, family member 1; CLCA1; CLCRG1; FLJ95147; GOB5; hCaCC-1; HCLCA1