Human CLEC-1 Antibody

Catalog #: AF1704 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1704
AF1704-SP

All CLEC-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human CLEC-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC-1
Gln77-Asp280
Accession # Q8NC01

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CLEC‑1 (Catalog # 1704-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-1

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC‑1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26%‑36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Pyz, E. et al. (2006) Ann. Med. 38:242.
Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.

Long Name

C-type Lectin-like Receptor-1

Entrez Gene IDs

51267 (Human); 243653 (Mouse)

Alternate Names

CLEC1; CLEC-1; CLEC1A; CLEC1C-type lectin domain family 1 member A; C-type lectin domain family 1, member A; C-type lectin-like receptor 1; C-type lectin-like receptor-1; MGC34328