Human Coagulation Factor III/Tissue Factor Antibody

Catalog #: MAB23391
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB23391
MAB23391-SP

Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry.

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All Coagulation Factor III/Tissue Factor Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no
cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.

Source

Monoclonal Mouse IgG₁ Clone # 323519

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
Gly34-Glu251
Accession # P13726

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA)

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB23391) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor, with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).

References

Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
Morrissey, J.H. et al. (1987) Cell 50:129.
Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Alternate Names

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Product Datasheets

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Related Research Areas

Citation for Human Coagulation Factor III/Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1