Home / Coagulation Factor III/Tissue Factor / Human Coagulation Factor III/
Tissue Factor Antibody

Human Coagulation Factor III/
Tissue Factor Antibody

Catalog #: AF2339
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2339
AF2339-SP
Detection of Human Coagulation Factor III/Tissue Factor by Western Blot.
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Product Details
Citations (7)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Coagulation Factor III/
Tissue Factor Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
Gly34-Glu251
Accession # P13726
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
A431 human epithelial carcinoma cell line
Flow Cytometry
2.5 µg/106 cells
U937 human histiocytic lymphoma cell line treated with LPS
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Coagulation Factor III/Tissue Factor by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Coagulation Factor III/Tissue Factor at approximately 48 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

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Citations for Human Coagulation Factor III/
Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin C
    Authors: C Mwase, TN Phung, MJ O'Sullivan, JA Mitchel, M De Marzio, A K?l?ç, ST Weiss, JJ Fredberg, JA Park
    Cells, 2022-01-13;11(2):.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Western Blot
  2. PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose Availability
    Authors: TLR Humphries, K Shen, A Iyer, DW Johnson, GC Gobe, D Nikolic-Pa, DP Fairlie, DA Vesey
    International Journal of Molecular Sciences, 2021-07-14;22(14):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood Clotting
    Authors: A Iyer, TLR Humphries, EP Owens, KN Zhao, PP Masci, DW Johnson, D Nikolic-Pa, GC Gobe, DP Fairlie, DA Vesey
    Frontiers in Physiology, 2021-03-10;12(0):615428.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The Low Molecular Weight Heparin Tinzaparin Attenuates Platelet Activation in Terms of Metastatic Niche Formation by Coagulation-Dependent and Independent Pathways
    Authors: LM Gockel, JM Ponert, S Schwarz, M Schlesinge, G Bendas
    Molecules, 2018-10-24;23(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016-05-04;6(0):25171.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011-12-12;6(12):e28540.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  7. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
    Authors: Kelly OG, Chan MY, Martinson LA
    Nat. Biotechnol., 2011-07-31;29(8):750-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Tissue Factor Antibody

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Human Coagulation Factor III/Tissue Factor Antibody
By Zoia Levashova on 05/30/2018
Application: MSD assay Sample Tested: Citrate Plasma,EDTA Plasma,Serum Species: Cynomolgus Monkey and Human

Used as detection antibody after labeling with Sulfo-Tag according to manufacturer's protocol (Meso Scale Diagnostics LLC). Detection range – 1.5-25,000 pg/ml

Other Human Coagulation Factor III/Tissue Factor Antibody AF2339