Human Complement Component C1r Antibody

Catalog #: MAB1807 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1807
MAB1807-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C1r Antibody Summary

Species Reactivity

Human

Specificity

Detects human C1r in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) C1s or rhC1q R1 is observed. In Western blots, ths antibody recognizes rhC1r and the A/heavy chain.

Source

Monoclonal Mouse IgG_2B Clone # 269104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
Ser18-Asp705
Accession # NP_001724

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Complement Component C1r (Catalog # 1807-SE) under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Complement Component C1r (Catalog # 1807-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1r

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain.

References

Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
Journet A. and M. Tosi (1986) Biochem. J. 240:783.

Entrez Gene IDs

715 (Human)

Alternate Names

C1r; complement C1r subcomponent; Complement component 1 subcomponent r; complement component 1, r subcomponent; Complement Component C1r; EC 3.4.21; EC 3.4.21.41