Human Complement Factor D/Adipsin Antibody

Name: Human Complement Factor D/Adipsin Antibody
Brand: R&D Systems
SKU: MAB18241
Rating: 5 (1 reviews)

Catalog #: MAB18241 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18241-SP
MAB18241-500
MAB18241-100

All Complement Factor D/Adipsin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor D/Adipsin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Factor D in ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 255719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor D
Ile26-Ala253
Accession # P00746

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Human Complement Factor D/Adipsin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Complement Factor D/Adipsin Biotinylated Antibody (Catalog # BAF1824)

Standard: Recombinant Human Complement Factor D Protein, CF (Catalog # 1824-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

References

White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
Taylor, F.R. et al. (1999) Biochemistry 38:2849.
Kim, S. et al. (1995) J. Biol. Chem. 270:24399.

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Alternate Names

Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN