Human Contactin-3 Antibody Summary
Pro26-Pro992
Accession # Q9P232
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Contactin-3 antibody in Human Brain by Immunohistochemistry (IHC-P). Contactin-3 antibody in Human Brain by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/Contactin-3_AF7125_Immunohistochemistry_11519.jpg)
Contactin‑3 in Human Brain. Contactin-3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7125) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-3
Contactin-3, also known as BIG-1, CNTN3 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
- Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
- Yoshihara, Y. et al. (1994) Neuron 13:415.
- Kamei, Y. et al. (2000) Genomics 69:113.
- Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Contactin-3 Antibody
There are currently no reviews for this product. Be the first to review Human Contactin-3 Antibody and earn rewards!
Have you used Human Contactin-3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image