Human Creatine Kinase MM/CKMM Antibody

Catalog #: MAB5564 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5564
MAB5564-SP

Detection of Human Creatine Kinase MM/CKMM by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Creatine Kinase MM/CKMM Antibody Summary

Species Reactivity

Human

Specificity

Detects human Creatine Kinase, Muscle/CKMM in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 492731

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Creatine Kinase MM/CKMM by Western Blot. Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/ CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase MM/CKMM at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Creatine Kinase MM/CKMM by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/ CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Creatine Kinase MM/CKMM

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Long Name

Creatine Kinase, Muscle

Entrez Gene IDs

1158 (Human); 12715 (Mouse); 24265 (Rat)

Alternate Names

CKM; CKMM; CKMMcreatine kinase M-type; Creatine Kinase Isoenzyme; Creatine kinase M chain; Creatine Kinase MM; creatine kinase, muscle; creatine kinase-M; EC 2.7.3; EC 2.7.3.2; M-CK