Human Creatine Kinase MM/CKMM Antibody

Catalog #: G-113-C Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
G-113-C

Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Creatine Kinase MM/CKMM Antibody Summary

Species Reactivity

Human

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM

Formulation

Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and Sodium Azide with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Simple Western

5 µg/mL

Human Heart

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western

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Detection of Human Creatine Kinase MM/CKMM by Simple Western^TM. Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/CKMM at approximately 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Creatine Kinase MM/CKMM Antigen Affinity-purified Polyclonal Antibody (Catalog # G-113-C). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Creatine Kinase MM/CKMM

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Long Name

Creatine Kinase, Muscle

Entrez Gene IDs

1158 (Human); 12715 (Mouse); 24265 (Rat)

Alternate Names

CKM; CKMM; CKMMcreatine kinase M-type; Creatine Kinase Isoenzyme; Creatine kinase M chain; Creatine Kinase MM; creatine kinase, muscle; creatine kinase-M; EC 2.7.3; EC 2.7.3.2; M-CK