Human CTRP1/C1qTNF1 Antibody

Catalog #: MAB10445 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10445-100
MAB10445-SP

Detection of Human CTRP1/C1qTNF1 by Western Blot.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CTRP1/C1qTNF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTRP1/C1qTNF1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 395520

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human CTRP1/C1qTNF1
Arg26-Pro281
Accession # Q9BXJ1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human CTRP1/C1qTNF1 by Western Blot. Western blot shows lysates of human heart (atrium) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CTRP1/C1qTNF1 Monoclonal Antibody (Catalog # MAB10445) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CTRP1/C1qTNF1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

CTRP1/C1qTNF1 in Human Heart. CTRP1/C1qTNF1 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CTRP1/C1qTNF1 Monoclonal Antibody (Catalog # MAB10445) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP1/C1qTNF1

C1qTNF1 (CTRP1) is an approximately 35 kDa member of the C1q family of secreted proteins and plays a role in energy metabolism and inflammation (1, 2). C1qTNF1 contains a collagen-like region and one C1q-like domain (3). Mature human C1qTNF1 shares 80% aa sequence identity with mouse and rat C1qTNF1. Circulating levels of C1qTNF1 are elevated in obesity, hypertension, and diabetes but can be decreased in the serum of diet-induced obese mice (4-6). C1qTNF1 expression is up-regulated in atherosclerotic plaques or adipose tissue by oxidized LDL or inflammatory cytokines (3, 7, 8). In turn, it induces the expression of inflammatory cytokines (7, 9) and the up-reglation of adhesion proteins on vascular endothelial cells (8). Systemically administered C1qTNF1, in contrast, can limit tissue damage following myocardial infarction (9). In skeletal muscle, C1qTNF1 promotes fatty acid oxidation, energy expenditure, insulin sensitivity, and glucose uptake and glycolysis (6, 10). It also induces the proliferation of immature chondrocytes (11) and aldosterone synthesis in the adrenal cortex (4). R&D Systems in-house testing indicates that C1qTNF1 binds to BAI3, consistent with the reported interactions between BAI3 and C1qL proteins (12).

References

Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
Seldin, M.M. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.
Kim, K.-Y. et al. (2006) FEBS Lett. 580:3953.
Jeon, J.H. et al. (2008) FASEB J. 22:1502.
Xin, Y. et al. (2014) Endocr. J. 61:841.
Peterson, J. M. et al. (2012) J. Biol. Chem. 287:1576.
Wang, X.Q. et al. (2016) Atherosclerosis 250:38.
Lu, L. et al. (2016) Eur. Heart J. 37:1762.
Yuasa, D. et al. (2016) FASEB J. 30:1065.
Han, S. et al. (2016) J. Nutr. Biochem. 27:43.
Akiyama, H. et al. (2013) Mol. Cell. Endocrinol. 369:63.
Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

Long Name

C1q And Tumor Necrosis Factor Related Protein 1

Entrez Gene IDs

114897 (Human); 56745 (Mouse); 303701 (Rat)

Alternate Names

C1q and tumor necrosis factor related protein 1; C1qTNF1; CTRP1; CTRP1G protein-coupled receptor-interacting protein; FLJ90694; G protein coupled receptor interacting protein; GIPcomplement C1q tumor necrosis factor-related protein 1; ZSIG37