Human CTRP3/C1qTNF3/CORS26 Antibody

Catalog #: MAB7925 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7925-100
MAB7925-SP

Human CTRP3/C1qTNF3/CORS26 ELISA Standard Curve.

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Human CTRP3/C1qTNF3/CORS26 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTRP3/C1qTNF3/CORS26 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 860115

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human CTRP3/C1qTNF3/CORS26
Gln23-Lys246
Accession # Q9BXJ4-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody(Catalog # MAB79251).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CTRP3/C1qTNF3/CORS26 DuoSet ELISA Kit (Catalog # DY7925-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human CTRP3/C1qTNF3/CORS26 ELISA Standard Curve. Recombinant Human CTRP3/C1qTNF3/CORS26 protein was serially diluted 2-fold and captured by Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody (Catalog # MAB7925) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CTRP3/C1qTNF3/CORS26 Monoclonal Antibody(Catalog # MAB79251) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP3/C1qTNF3/CORS26

CTRP3 (Complement C1q TNF-related Protein 3/C1qTNF3) also known as CORS-26 (Collagenous Repeat-containing Sequence of 26 kDa protein), Cartonectin and Cartducin, is a 30-32 kDa, secreted member of the C1q and TNF-related (CTRP) superfamily of molecules (1). The mature protein is 224 aa in length. It contains an N-terminal collagen-like domain followed by a C-terminal globular region. Human CTRP3 shares 99% aa sequence identity with the mouse CTRP3 (2). Like other CTRP members, CTRP3 has a trimeric structure and can assemble into hexameric or higher order molecular forms (3). It is expressed by a variety of cells, including adipocytes, cartilage, fibroblasts, monocytes and proliferating chondrocytes (4). The inflammatory effects of LPS, TLR-4 and fatty acids have been shown to be inhibited by CTRP3 in adipocytes and monocytes (5). In mouse models, CTRP3 has been shown to lower glucose levels and decrease gluconeogenic gene expression (6). Inhibition of 3T3-L1 pre-adipocyte differentiation to adipocytes is associated with CTRP3 treatment, demonstrating potential anti-obesity effects (7). Treatment with CTRP3 results in the proliferation of skeletal muscle C2C12 cells and inhibition of C2C12 myotube differentiation, mediated by the ERK pathway (8). Due to the variety of functions in metabolism and inflammation, CTRP3 is a potential new target of type 2 diabetes treatment.

References

Wong, G.W. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:10302.
Schaffler A. et al. (2003). Biochim Biophys Acta.1630:123.
Suzuki S. et al. (2007) FEBS Lett. 581: 809.
Weigert J. et al. (2005) FEBS Lett. 579: 5565.
Kopp, A. et al. (2010) Endocrinology. 151:5267.
Peterson J. M. et al. (2010) J Biol Chem. 285:39691.
Nishimoto, H. et al. (2017) Cell Bio Int. 41:197.
Otani M. et al. (2015) Mol Cell Biochem. 409:271.

Long Name

C1q and Tumor Necrosis Factor-related Protein 3

Entrez Gene IDs

114899 (Human); 81799 (Mouse)

Alternate Names

C1ATNF3; C1q and tumor necrosis factor related protein 3; C1qTNF3; Cartducin; cartonectin; collagenous repeat-containing sequence of 26-kDa; complement C1q tumor necrosis factor-related protein 3; complement-c1q tumor necrosis factor-related protein 3; Corcs; Cors; CORS26; Cors-26; CTRP3; CTRP32310005P21Rik; FLJ37576; Secretory protein CORS26