Human CXCL4/PF4 Antibody Summary
Glu32-Ser101
Accession # P02776.2
Applications
Human CXCL4/PF4 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL4/PF4
CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C‑terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
- Poncz, M. et al. (1987) Blood 69:219.
- Scheuerer, B. et al. (2000) Blood 95:1158.
- Perollet, C. et al. (1998) Blood 91:3289.
- Petersen, F. et al. (1998) J. Immunol. 161:4347.
- Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
- Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Product Datasheets
Citations for Human CXCL4/PF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
Authors: JC de Vries, AD Barendrech, CC Clark, RT Urbanus, P Boross, S de Maat, C Maas
Sci Rep, 2019-12-04;9(1):18316.
Species: Human
Sample Types: Citrated Blood
Applications: IHC -
2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia
Authors: E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert
Blood Adv, 2018-04-10;2(7):754-761.
Species: Mouse
Sample Types: Protein
Applications: ELISA Capture -
Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction
Authors: JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch
Pharmaceuticals (Basel), 2017-03-29;10(2):.
Species: Human
Sample Types: Platelet Rich Plasma (PRP)
Applications: ELISA Development (Capture) -
Granule exocytosis is required for platelet spreading: differential sorting of alpha-granules expressing VAMP-7.
Blood, 2012-05-15;120(1):199-206.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Up-regulation of platelet activation in hemophilia A.
Authors: van Bladel ER, Roest M
Haematologica, 2011-03-21;0(0):.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study.
Authors: Snoep JD, Roest M, Barendrecht AD
J. Thromb. Haemost., 2010-01-30;8(5):906-13.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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This antibody was used as the capture with BAF795 as the detection in an ELISA. While functional it was difficult to get a broad dynamic range on the ELISA with this antibody.
