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Human CXCR4 Antibody

Catalog #: MAB170
42 Citations Datasheet / COA / SDS
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MAB170
MAB170-SP
Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (42)
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Human CXCR4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR4.
Source
Monoclonal Mouse IgG2A Clone # 12G5
Purification
Protein A or G purified from ascites
Immunogen
CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
Accession # P61073
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
see below
CyTOF-reported
Nair, N. et al. (2016) Mucosal Immunol. 9: 68. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Neutralization Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutrali-zation by Human CXCR4 Antibody. View Larger

Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutrali-zation by Human CXCR4 Antibody. Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND50 is typically 0.3-1.2 µg/mL.

Flow Cytometry View Larger

Detection of CXCR4 in Jurkat cells vs K562 by Flow Cytometry Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 Monoclonal Antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.
Long Name
C-X-C Motif Chemokine Receptor 4
Entrez Gene IDs
7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names
CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Citations for Human CXCR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. An Evaluation of CXCR4 Targeting with PAMAM Dendrimer Conjugates for Oncologic Applications
    Authors: WG Lesniak, BB Azad, S Chatterjee, A Lisok, MG Pomper
    Pharmaceutics, 2022-03-16;14(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Stimulated Expression of CXCL12 in Adrenocortical Carcinoma by the PPARgamma Ligand Rosiglitazone Impairs Cancer Progression
    Authors: G Cantini, L Fei, L Canu, E Lazzeri, M Sottili, M Francalanc, ML Angelotti, G De Filpo, T Ercolino, S Gelmini, M Mangoni, G Nesi, C Hantel, M Mannelli, M Maggi, M Luconi
    Journal of personalized medicine, 2021-10-27;11(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis
    Authors: M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane
    PloS Pathogens, 2021-04-19;17(4):e1009526.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  4. CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells
    Authors: C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala
    Cell Death Dis, 2019-07-22;10(8):562.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer
    Authors: S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu
    Cancer Res., 2019-04-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. GRK2 mediates TCR-induced transactivation of CXCR4 and TCR-CXCR4 complex formation which drives PI3K?/PREX1 signaling and T cell cytokine secretion
    Authors: BA Dinkel, KN Kremer, MR Rollins, MJ Medlyn, KE Hedin
    J. Biol. Chem., 2018-07-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4
    Authors: SC Mills, L Howell, A Beekman, L Stokes, A Mueller
    Cell. Signal., 2017-10-16;42(0):88-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CXCL12–CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation
    Authors: Hirohito Minami, Keiki Nagaharu, Yoshiki Nakamori, Kohshi Ohishi, Naoshi Shimojo, Yuki Kageyama et al.
    The Journal of Immunology
  9. ?20 IFITM2 differentially restricts X4 and R5 HIV-1
    Authors: WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang
    Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  10. CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment
    Authors: Y Han, C Wu, J Wang, N Liu
    Tumour Biol., 2017-05-01;39(5):1010428317701.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices
    Proc Natl Acad Sci USA, 2016-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  12. HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1?
    Mol Cell Biol, 2016-07-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  13. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
    Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
    J Biol Chem, 2016-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia.
    Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C
    Cancer Res, 2015-08-17;75(19):4153-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
    Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
    PLoS ONE, 2015-03-16;10(3):e0120069.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
    Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
    Clin Cancer Res, 2015-02-23;21(12):2840-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
    Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
    Stem Cells Transl Med, 2014-12-10;4(1):44-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. CXCR7 controls competition for recruitment of beta-arrestin 2 in cells expressing both CXCR4 and CXCR7.
    Authors: Coggins N, Trakimas D, Chang S, Ehrlich A, Ray P, Luker K, Linderman J, Luker G
    PLoS ONE, 2014-06-04;9(6):e98328.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. CXCR4, but not CXCR7, Discriminates Metastatic Behavior in Non–Small Cell Lung Cancer Cells
    Authors: Young H. Choi, Marie D. Burdick, Brett A. Strieter, Borna Mehrad, Robert M. Strieter
    Molecular Cancer Research
  20. Transcriptional regulation of CXCR4 in prostate tumor cells: Significance of TMPRSS2-ERG fusions.¶
    Authors: Rajareddy Singareddy, Louie Semaan, M. Katie Conley-LaComb, Jason St. John, Katelyn Powell, Matthew Iyer et al.
    Molecular Cancer Research
  21. An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration.
    Authors: Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B
    J Biol Chem, 2013-08-21;288(40):28656-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Consequences of ChemR23 Heteromerization with the Chemokine Receptors CXCR4 and CCR7
    Authors: Cédric de Poorter, Kevin Baertsoen, Vincent Lannoy, Marc Parmentier, Jean-Yves Springael
    PLoS ONE
  23. Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes
    Authors: Dustin N D Lippert, John A Wilkins
    BMC Immunology
  24. The miR 302-367 cluster drastically affects self-renewal and infiltration properties of glioma-initiating cells through CXCR4 repression and consequent disruption of the SHH-GLI-NANOG network
    Authors: M Fareh, L Turchi, V Virolle, D Debruyne, F Almairac, S de-la-Forest Divonne et al.
    Cell Death & Differentiation
  25. In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors
    Authors: Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra
    Experimental and Molecular Medicine
  26. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011-04-11;17(11):3619-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC-P
  28. Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.
    Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
    Exp. Hematol., 2008-06-17;36(9):1121-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells.
    Authors: Kaminski A, Ma N, Donndorf P, Lindenblatt N, Feldmeier G, Ong LL, Furlani D, Skrabal CA, Liebold A, Vollmar B, Steinhoff G
    Lab. Invest., 2007-11-26;88(1):58-69.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  30. Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium.
    Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW
    Cancer Res., 2007-05-01;67(9):4244-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007-01-01;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
    Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
    AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
    Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
    J. Cell. Physiol., 2006-05-01;207(2):364-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Skin homing of Sezary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV.
    Authors: Narducci MG, Scala E, Bresin A, Caprini E, Picchio MC, Remotti D, Ragone G, Nasorri F, Frontani M, Arcelli D, Volinia S, Lombardo GA, Baliva G, Napolitano M, Russo G
    Blood, 2005-10-04;107(3):1108-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  35. Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells.
    Authors: Lawrence DM, Durham LC, Schwartz L, Seth P, Maric D, Major EO
    J. Virol., 2004-07-01;78(14):7319-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  36. CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice.
    Authors: Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T
    Cancer Res., 2004-04-15;64(8):2817-24.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P, Neutralization
  37. Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.
    Authors: Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F
    Stem Cells, 2004-01-01;22(6):1015-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia?
    Authors: Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP
    Br. J. Haematol., 2002-11-01;119(2):579-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells.
    Authors: Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG
    J. Leukoc. Biol., 2002-10-01;72(4):837-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
    Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
    Blood, 2002-10-01;100(7):2321-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002-06-15;168(12):6244-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  42. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms.
    Authors: Rahimi Massod, George Jessica, Tang Careen et al.
    International Journal of Cancer

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