Human CXCR5 PE-conjugated Antibody
Human CXCR5 PE-conjugated Antibody Summary
Met1-Phe372
Accession # P32302
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR5
CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.
Product Datasheets
Citations for Human CXCR5 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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CXCR5 CAR-T cells simultaneously target B cell non-Hodgkin's lymphoma and tumor-supportive follicular T helper cells
Authors: M Bunse, J Pfeilschif, J Bluhm, M Zschummel, JJ Joedicke, A Wirges, H Stark, V Kretschmer, M Chmielewsk, W Uckert, H Abken, J Westermann, A Rehm, UE Höpken
Nature Communications, 2021-01-11;12(1):240.
Species: Human, Xenograft
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Follicular T Cells from smB(-) Common Variable Immunodeficiency Patients Are Skewed Toward a Th1 Phenotype
Authors: V Cunill, A Clemente, N Lanio, C Barceló, V Andreu, J Pons, JM Ferrer
Front Immunol, 2017-02-27;8(0):174.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
PLoS ONE, 2015-03-16;10(3):e0120069.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization.
Authors: Martinez-Martin N, Viejo-Borbolla A, Martin R, Blanco S, Benovic J, Thelen M, Alcami A
Nat Commun, 2015-01-27;6(0):6163.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
J Immunol, 2014-02-19;192(6):2634-42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
PLoS ONE, 2014-01-31;9(1):e87131.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
Authors: Badr G, Lefevre EA, Mohany M
PLoS ONE, 2011-09-01;6(9):e23741.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
PLoS ONE, 2011-08-03;6(8):e22848.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
Clin. Exp. Immunol., 2010-11-19;163(2):215-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010-02-17;184(6):2886-98.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
Immunology, 2009-08-01;127(4):549-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
Br. J. Cancer, 2008-09-16;99(6):930-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
Blood, 2008-09-09;112(12):4401-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
J. Exp. Med., 2008-07-14;205(8):1797-805.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
J. Neuroimmunol., 2008-06-09;199(1):35-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
Cancer Res., 2008-02-01;68(3):790-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
Blood, 2008-01-02;111(5):2744-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia.
Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA
Blood, 2007-07-25;110(9):3316-25.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
J. Immunol., 2007-03-01;178(5):2872-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
Int. J. Cancer, 2006-05-01;118(9):2147-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
Blood, 2004-09-28;105(3):931-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
J. Leukoc. Biol., 2003-05-01;73(5):584-90.
Species: Human
Sample Types: CSF
Applications: Flow Cytometry
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The PBMCs were transduced with CXCR5 resulting in a high population expressing CXCR5 PE
Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100