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Human Cystatin SN Antibody

Catalog #: MAB1285
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1285
MAB1285-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cystatin SN Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cystatin SN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, D, S, SA, E/M, rhFetuin A, rhFetuin B, rhHPRG, or rhThrombin is observed.
Source
Monoclonal Mouse IgG2B Clone # 213139
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin SN
Trp21-Ser141 (predicted)
Accession # P01037
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cystatin SN (Catalog # 1285-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cystatin SN (Catalog # 1285-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin SN

Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).

References
  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Baron, A.C. et al. (1999) Oral Dis. 5:344.
  3. Baron, A. et al. (1999) Oral Dis. 5:234.
Entrez Gene IDs
1469 (Human)
Alternate Names
CST1; cystain-SA-I; cystatin 1; cystatin SA-I; Cystatin SN; cystatin-1; cystatin-SN; Salivary cystatin-SA-1; type 2 family

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Citation for Human Cystatin SN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Murine monoclonal antibody which can distinguish cystatins SA1 and SA2.
    Authors: Ito T, Komiya-Ito A, Okuda K, Minaguchi K, Saitoh E, Yamada S, Kato T
    Mol. Immunol., 2005-01-08;42(10):1259-63.
    Species: Human
    Sample Types: Recombinant Protein, Saliva
    Applications: ELISA Development, Western Blot

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