Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody

Name: Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody
Brand: R&D Systems
SKU: MAB5959
Rating: 5 (1 reviews)

Catalog #: MAB5959 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5959
MAB5959-SP

Cytosolic Sulfotransferase 1B1/SULT1B1 in Human Liver.

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Human Cytosolic Sulfotransferase 1B1/SULT1B1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytosolic Sulfotransferase 1B1/SULT1B1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1C1, or rhSULT1E1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 724429

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1B1/SULT1B1
Leu2-Ile296
Accession # O43704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Cytosolic Sulfotransferase 1B1/SULT1B1 in Human Liver. Cytosolic Sulfotransferase 1B1/SULT1B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Cytosolic Sulfotransferase 1B1/SULT1B1 Monoclonal Antibody (Catalog # MAB5959) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1B1/SULT1B1

Cytosolic Sulfotransferases are a family of phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1‑3). They have important functions in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are distributed throughout the body and serve to inactivate and increase water-solubility of xenobiotics and therapeutic drugs. Cytosolic sulfotransferases are distinct from Golgi resident sulfotransferases by lacking N-terminal signal-anchorage domains and residing only in the cytoplasm. SULT1B1 is primarily expressed in the liver, peripheral blood leukocytes, colon, spleen and small intestine and can sulfate thyroid hormones and small phenols (4). Human SULT1B1 shares 72% amino acid sequence identity with mouse SULT1B1.

References

Falany, C, N. (1997) FASEB J. 11:206.
Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
Allali-Hassani, A. et al. (2007) PLoS Biol. 5:e97.
Wang, J. et al. (1998) Mol. Pharmacol. 53:274.

Entrez Gene IDs

27284 (Human); 56362 (Mouse); 64305 (Rat)

Alternate Names

Cytosolic Sulfotransferase 1B1; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.1; EC 2.8.2.4; MGC13356; ST1B1; ST1B2; ST1B2sulfotransferase family cytosolic 1B member 1; Sulfotransferase 1B1; Sulfotransferase 1B2; sulfotransferase family, cytosolic, 1B, member 1; SULT1B1; SULT1B2; Thyroid hormone sulfotransferase