Human DDR2 Antibody Summary
Gln24-Arg399
Accession # Q16832
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human DDR2 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with Calyculin A for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for DDR2 at approximately 139 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human DDR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2538) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDR2
DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).
- Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
- Karn, T. et al. (1993) Oncogene 8:3433.
- Vogel, W. et al. (1997) Mol. Cell 1:13.
- Leitinger, B. (2003) J. Biol. Chem. 278:16761.
- Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
- Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
- Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
- Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
- Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
- Xu, L. et al. (2005) J. Biol. Chem. 280:548.
- Wang, J. et al. (2002) J. Autoimmun. 19:161.
- Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.
Product Datasheets
Citations for Human DDR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration
Authors: T Han, P Mignatti, SB Abramson, M Attur
PLoS ONE, 2020-04-24;15(4):e0231501.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Chain alignment of collagen I deciphered using computationally designed heterotrimers
Authors: AA Jalan, D Sammon, JD Hartgerink, P Brear, K Stott, SW Hamaia, EJ Hunter, DR Walker, B Leitinger, RW Farndale
Nat. Chem. Biol., 2020-01-06;0(0):.
Species: Human
Sample Types: Cell Lyates
Applications: Western Blot -
Discoidin Domain Receptor 2 Mediates Collagen-Induced Activation of Membrane-Type 1 Matrix Metalloproteinase in Human Fibroblasts
Authors: I Majkowska, Y Shitomi, N Ito, NS Gray, Y Itoh
J. Biol. Chem, 2017-03-07;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
Authors: Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, Paul A, Esposito E, Gridley S, Leitinger B, Naegle K, Huang P
Biochem J, 2013-09-15;454(3):501-13.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase.
Biochem Biophys Res Commun, 2012-11-03;429(1):39-44.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot -
Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice.
Authors: Kawai I, Hisaki T, Sugiura K, Naito K, Kano K
Biochem Biophys Res Commun, 2012-09-26;427(3):611-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1.
Authors: Xu H, Raynal N, Stathopoulos S, Myllyharju J, Farndale RW, Leitinger B
Matrix Biol., 2010-10-28;30(1):16-26.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human DDR2 Antibody
There are currently no reviews for this product. Be the first to review Human DDR2 Antibody and earn rewards!
Have you used Human DDR2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
