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Human Dectin-1/CLEC7A Antibody

Catalog #: MAB1859
27 Citations 2 Reviews Datasheet / COA / SDS
Clone 259931 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1859
MAB1859-SP
Dectin‑1/CLEC7A in Human Dendritic Cells.
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Citations (27)
FAQs
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dectin-1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 259931
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A Thr66-Met201 Accession # NP_072092
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Dectin-1/CLEC7A antibody in Human Dendritic Cells by Immunocytochemistry (ICC). View Larger

Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4 + T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

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Citations for Human Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity
    Authors: Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2
    Authors: S Haji, T Ito, C Guenther, M Nakano, T Shimizu, D Mori, Y Chiba, M Tanaka, SK Mishra, JA Willment, GD Brown, M Nagae, S Yamasaki
    Elife, 2022-12-08;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis
    Authors: N Rochereau, E Michaud, L Waeckel, M Killian, R Gayet, R Goguyer-De, X Roblin, G Biolley, B Corthésy, S Paul
    Cell Reports, 2021-11-16;37(7):110006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021-07-20;36(3):109392.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  6. Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination
    Authors: JC Silva, NC Rodrigues, GA Thompson-S, VS Muniz, JS Neves, RT Figueiredo
    J. Leukoc. Biol., 2019-09-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis
    Authors: JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic
    J Immunol Res, 2019-02-06;2019(0):1529189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Cellular metabolism constrains innate immune responses in early human ontogeny
    Authors: B Kan, C Michalski, H Fu, HHT Au, K Lee, EA Marchant, MF Cheng, E Anderson-B, M Aharoni-Si, P Tilley, RG Mirmira, CJ Ross, DS Luciani, E Jan, PM Lavoie
    Nat Commun, 2018-11-16;9(1):4822.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
    Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
    PLoS Biol., 2018-04-18;16(4):e2005504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans
    Authors: M Swidergall, NV Solis, MS Lionakis, SG Filler
    Nat Microbiol, 2017-11-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  11. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
    Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
    Int J Mol Sci, 2017-03-13;18(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016-11-29;11(11):e0167410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
    Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
    J Immunol, 2015-12-30;196(3):1272-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
    Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
    Eur J Immunol, 2015-12-02;46(2):455-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
    Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
    J Immunol, 2015-06-29;195(4):1723-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015-03-20;10(3):e0120948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  17. Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk.
    Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
    Eur J Immunol, 2014-10-27;44(12):3729-40.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  18. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014-06-14;69(1):102-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Podosomes of dendritic cells facilitate antigen sampling.
    Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
    J Cell Sci, 2014-01-14;127(0):1052-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
    Authors: Lefevre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre J, Auwerx J, Neyrolles O, Pipy B, Coste A
    Immunity, 2013-05-16;38(5):1038-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
    Authors: Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, Gow N, van der Meer J, Netea M, Quintin J
    J Immunol, 2013-03-08;190(8):4129-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012-12-11;7(12):e50589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
    Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
    Eur. J. Immunol., 2012-06-01;42(6):1512-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  24. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome.
    Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
    Nat. Immunol., 2012-01-22;13(3):246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
    Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
    J. Leukoc. Biol., 2011-06-07;90(3):599-611.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  26. Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
    Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
    PLoS Pathog., 2011-01-20;7(1):e1001259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human Dectin-1/CLEC7A Antibody
By Anonymous on 11/28/2020
Application: Flow Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.

Flow Cytometry Human Dectin-1/CLEC7A Antibody MAB1859
Human Dectin-1/CLEC7A Antibody
By Anonymous on 10/13/2020
Application: Flow Sample Tested: macrophages Species: Human

Detection of human Dectin­1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin­1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fcγ fragment APC antibody.

Flow Cytometry Human Dectin-1/CLEC7A Antibody MAB1859