Human Desmoglein-1 Antibody Summary
Glu50-His545
Accession # Q02413
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Desmoglein‑1 in Human Skin Hyperplasia. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocyte membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-1
Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters.
- Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
- Elias, P. et al. (2001) J. Cell Biol. 153:243.
Product Datasheets
Citations for Human Desmoglein-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection
Authors: T Sumitomo, Y Mori, Y Nakamura, M Honda-Ogaw, S Nakagawa, M Yamaguchi, H Matsue, Y Terao, M Nakata, S Kawabata
Front Cell Infect Microbiol, 2018-01-24;8(0):10.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens.
Authors: Chernyavsky AI, Arredondo J, Kitajima Y, Sato-Nagai M, Grando SA
J. Biol. Chem., 2007-03-07;282(18):13804-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Reconstructed human epidermis tissues were embedded in paraffin. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. A 1:100 dilution of the antibody was used (overnight incubation at 4°C).
Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein)
Antibody dilution 1:50, works also at 1:100 dilution
