Human Dkk-1 Antibody

Name: Human Dkk-1 Antibody
Brand: R&D Systems
SKU: MAB1096
Rating: 5 (1 reviews)

Catalog #: MAB1096
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1096-050
MAB1096-500
MAB1096-SP

All Dkk-1 Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dkk-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.

Source

Monoclonal Mouse IgG₁ Clone # 141119

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Dkk‑1 (Catalog # 5439-DK)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Long Name

Dickkopf-1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Alternate Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Dkk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Pharmacokinetic immunoassay methods in the presence of soluble target.
Authors: Verch T, Chilewski S, Bouaraphan S, Yarovoi H, Yin KC, Chen D, Washabaugh MW
J. Immunol. Methods, 2010-08-07;361(1):75-81.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.
Authors: Liu CC, Pearson C, Bu G
J. Biol. Chem., 2009-03-31;284(22):15299-307.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Production of Wnt inhibitors by myeloma cells: potential effects on canonical Wnt pathway in the bone microenvironment.
Authors: Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Donofrio G, Bonomini S, Sala R, Mangoni M, Rizzoli V
Cancer Res., 2007-08-15;67(16):7665-74.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot