Human DLL1 Antibody

Catalog #: AF1818
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1818
AF1818-SP

Detection of DLL1 in T98G Human Cell Line by Flow Cytometry.

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All DLL1 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human DLL1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human DLL4 and recombinant mouse DLL4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLL1
Ser22-Gly540
Accession # AAG09716

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Flow Cytometry

1 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DLL1 in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Goat Anti-Human DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1818, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References

Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
Karanu, F.N. et al. (2001) Blood 97:1960.
Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
Takahashi, Y. et al. (2003) Development 130:4259.
Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
Kiernan, A.E. et al. (2005) Development 132:4353.
Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. USA 104:15454.
Limbourg, A. et al. (2007) Circ. Res. 100:363.
Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Long Name

Delta-like 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Alternate Names

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Product Datasheets

Product Datasheet

COA

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Citations for Human DLL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Selective CDK9 inhibition overcomes TRAIL resistance by concomitant suppression of cFlip and Mcl-1.
Authors: Lemke J, von Karstedt S, Abd El Hay M, Conti A, Arce F, Montinaro A, Papenfuss K, El-Bahrawy M, Walczak H
Cell Death Differ, 2013-12-20;21(3):491-502.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Roles of Pofut1 and O-fucose in mammalian Notch signaling.
Authors: Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
J. Biol. Chem., 2008-03-17;283(20):13638-51.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry