Human Dopamine D1R/DRD1 Antibody

Catalog #: MAB8276
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8276
MAB8276-SP

Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dopamine D1R/DRD1 Antibody Summary

Species Reactivity

Human

Specificity

Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 887438

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP was stained with either (A) Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunohistochemistry

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Dopamine D1 R/DRD1 in Human Brain. Dopamine D1 R/DRD1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine D1R/DRD1

Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.

Long Name

Dopamine D1 Receptor

Entrez Gene IDs

1812 (Human); 13488 (Mouse); 24316 (Rat)

Alternate Names

D(1A) dopamine receptor; D1DR; DADR; Dopamine D1 R; Dopamine D1 receptor; Dopamine D1R; dopamine receptor D1; DRD1; DRD1A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Dopamine D1R/DRD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dopamine receptors D1 and D2 show prognostic significance and potential therapeutic applications for endometrial cancer patients
Authors: Español, P;Rovira, R;Caruana, P;Luna-Guibourg, R;Soler, C;Teixeira, N;Rodríguez, F;Gallardo, A;Edwards, M;Porta, O;Gámez, M;Sánchez, O;Llurba, E;Corchero, JL;Céspedes, MV;
Gynecologic oncology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry