Human DPP9 Antibody Summary
Arg2-Leu892 (predicted)
Accession # Q1ZZB8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human DPP9 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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DPP9 in HeLa Human Cell Line. DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human DPP9 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for DPP9 at approximately 101 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB5419). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPP9
DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).
- Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
- Qi, S.Y. et al. (2003) Biochem. J. 373:179.
- Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
- Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
- Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
- Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
Product Datasheets
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