Human E-Selectin/P-Selectin (CD62E/P) PE-conjugated Antibody

Catalog #: FAB6169P Datasheet / COA / SDS

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FAB6169P

Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Cells by Flow Cytometry.

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All E-Selectin (CD62E)/P-Selectin (CD62P) Antibodies

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Human E-Selectin/P-Selectin (CD62E/P) PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Source

Monoclonal Mouse IgG₁ Clone # BBIG-E6 (13D5)

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) PE-conjugated Monoclonal Antibody (Catalog # FAB6169P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin (CD62E) and P-Selectin (CD62P) are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names

CD62E; ELAM; ELAM1; ESEL; Eselectin; E-Selectin/P-Selectin; LECAM2; PSelectin