Human EBI3 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 family that is composed of two chains, EBV-induced gene 3( EBI3), a 33 kDa glycosylated protein homologous to the p40 subunit of IL-12, and p28, a 28 kDa peptide analogous to the p35 subunit of IL-12. IL-27 has been found to be expressed by activated monocytes and monocyte-derived dendritic cells, astrocytes, adipocytes, CD4⁺ T cells, and macrophages. In human tissues, EBI3 and p28 coexpression has been observed in macrophages or macrophage-derived cells, and in endothelial cells. The IL-27 receptor complex comprises two chains, IL-27R (also called WSX-1 or TCCR) and gp130. IL-27 is the only known ligand for IL-27R, whereas gp130 is a receptor component of several cytokines of the IL-6 family. Functionally, IL-27 appears to be immunosuppressive. On T cells in mice, it induces IL-10 secretion while suppressing IL-17A and GM-CSF production. But the relationship between IL-27 and IL-17A may be complex. After IL-17A induction, IL-27 secretion occurs in unstimulated cells, suggesting IL-27 downregulates bystander activation in inflammatory conditions. EBI3 also complexes with p35 (IL-12) to form IL-35. The mature EBI3 sequences for human and mouse share 62% amino acid sequence identity.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• B Cell Markers
• IL-12 Family
• Regulatory T Cells (Tregs)
• Th1 Cells