Human ECE-1 Antibody

Catalog #: MAB1784
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1784
MAB1784-SP

All ECE-1 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ECE-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ECE-1 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ECE-2 or rhMMEL-2.

Source

Monoclonal Rat IgG₁ Clone # 303908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ECE-1
Gln90-Trp770
Accession # P42892

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human ECE‑1 (Catalog # 1784-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECE-1

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

References

Turner, A.J. et al. (2001) BioEssays 23:261.
Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
Schweizer, A. et al. (1997) Biochem. J. 328:871.
Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Long Name

Endothelin-converting Enzyme-1

Entrez Gene IDs

1889 (Human)

Alternate Names

EC 3.4.24; EC 3.4.24.71; ECE; ECE1; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ECE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Functional and molecular characterization of the endothelin system in retinal arterioles.
Authors: Hein TW, Ren Y, Yuan Z, Xu W, Somvanshi S, Nagaoka T, Yoshida A, Kuo L
Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(7):3329-36.
Species: Porcine
Sample Types: Cell Lysates
Applications: Western Blot