Human ECE-1 Antibody Summary
Gln90-Trp770
Accession # P42892
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ECE‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Human ECE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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ECE‑1 in Human Kidney. ECE‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ECE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ECE-1
Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
- Schweizer, A. et al. (1997) Biochem. J. 328:871.
- Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Product Datasheets
Citations for Human ECE-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors
Authors: Q Yu, K Li, A Zhao, M Wei, Z Huang, Y Zhang, Y Chen, L Ting, W Chuan, L Xu, Y Zhang, C Xu, F Liu
Toxicology and Applied Pharmacology, 2021-01-24;414(0):115420.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Retinal genes are differentially expressed in areas of primary versus secondary degeneration following partial optic nerve injury
Authors: W Chiha, CJ LeVaillant, CA Bartlett, AW Hewitt, PE Melton, M Fitzgerald, AR Harvey
PLoS ONE, 2018-02-09;13(2):e0192348.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Endothelin-converting enzymes degrade ?-synuclein and are reduced in dementia with Lewy bodies
Authors: JS Miners, S Love
J. Neurochem, 2017-03-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation.
Authors: Whyteside A, Turner A, Lambert D
PLoS ONE, 2014-01-31;9(1):e83260.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.
Authors: Zhao P, Canals M, Murphy J, Klingler D, Eriksson E, Pelayo J, Hardt M, Bunnett N, Poole D
J Biol Chem, 2013-08-02;288(36):25689-700.
Species: Mouse, Rat
Sample Types: Whole Tissue
Applications: IHC -
Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
Authors: Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS
J. Neurochem., 2010-07-30;115(1):47-57.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling.
Authors: Cottrell GS, Padilla BE, Amadesi S, Poole DP, Murphy JE, Hardt M, Roosterman D, Steinhoff M, Bunnett NW
J. Biol. Chem., 2009-06-16;284(33):22411-25.
Species: Mouse, Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins.
Authors: Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW
J. Cell Biol., 2007-11-26;179(5):981-97.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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