Human EDA/Ectodysplasin Biotinylated Antibody

Catalog #: BAM9221 Datasheet / COA / SDS

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BAM9221

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EDA/Ectodysplasin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human EDA/Ectodysplasin (EDA-A1 and EDA-A2) in ELISAs. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse EDA is observed, and no cross-reactivity with recombinant human (rh) TNF-alpha, rhXEDAR, or rhEDAR is observed.

Source

Monoclonal Mouse IgG_2B Clone # 174817

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EDA-A2/Ectodysplasin A2
Ala179-Ser389 (predicted)
Accession # NP_001005609

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human EDA-A2/Ectodysplasin A2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human EDA/Ectodysplasin Antibody (Catalog # MAB9222)

Standard: Recombinant Human EDA-A2 Protein (Catalog # 922-ED)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA/Ectodysplasin

The products of the Ectodysplasin A gene (EDA, also called Tabby) are type II transmembrane proteins that belong to the TNF superfamily. The EDA gene encodes two splice variants, designated EDA-A1 and EDA-A2, that differ only by the deletion of two amino acids in the extracellular domain of EDA-A2. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. The extracellular domains of human and mouse EDA-A2 share approximately 94% amino acid sequence identity.

Entrez Gene IDs

1896 (Human); 13607 (Mouse)

Alternate Names

ectodysplasin A; ED1; EDA; Tabby; XHED; XLHED