Human EGF Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 15 | 78.9 | 134 | 15 | 80 | 136 |
Standard Deviation | 0.4 | 2.5 | 4 | 0.9 | 4.5 | 6.8 |
CV% | 2.7 | 3.2 | 3 | 6 | 5.6 | 5 |
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 13.5 | 80 | 133 | 14.7 | 77 | 134 |
Standard Deviation | 0.6 | 2 | 2.7 | 0.9 | 3.3 | 6.1 |
CV% | 4.4 | 2.5 | 2 | 6.1 | 4.3 | 4.6 |
Recovery
The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=5) | 98 | 88-111 |
Citrate Plasma (n=10) | 104 | 89-115 |
EDTA Plasma (n=10) | 100 | 80-111 |
Heparin Plasma (n=10) | 96 | 79-108 |
Serum (n=10) | 97 | 80-106 |
Urine (n=1) | 102 | 97-108 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
For Cell Culture Supernate & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour. - Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human EGF Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Molecular signature of stem-like glioma cells (SLGCs) from human glioblastoma and gliosarcoma
Authors: Zechel, C;Loy, M;Wegner, C;Dahlke, E;Soetje, B;Baehr, L;Leppert, J;Ostermaier, JJ;Lueg, T;Nielsen, J;El beta ner, J;Willeke, V;Marzahl, S;Tronnier, V;Madany Mamlouk, A;
PloS one
Species: Human hepegivirus
Sample Types: Cell Culture Supernates
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Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing
Authors: V Sabrina, C Carmen, A Gloria, B Elisa, C Nico, B Marina, F Luigi, V Piera
International Journal of Molecular Sciences, 2022-09-14;23(18):.
Species: Human
Sample Types: Serum
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Predicting treatment response and clinicopathological findings in lupus nephritis with urine epidermal growth factor, monocyte chemoattractant protein-1 or their ratios
Authors: P Ngamjanyap, S Worawichaw, P Pisitkun, K Khiewngam, S Kantachuve, A Nongnuch, M Assanatham, N Sathirapon, C Kitiyakara
PLoS ONE, 2022-03-10;17(3):e0263778.
Species: Human
Sample Types: Urine
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A comparative study of pathogen inactivation technologies in human platelet lysate and its optimal efficiency in human placenta-derived stem cells culture
Authors: E Razani, HR Aghayan, S Alavi-Mogh, F Yari, A Gharehbagh, Z Sharifi
Journal of virological methods, 2022-01-29;0(0):114478.
Species: Human
Sample Types: Cell Lysates
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Oncogenic KRAS promotes growth of lung cancer cells expressing SLC3A2-NRG1 fusion via ADAM17-mediated shedding of NRG1
Authors: DH Shin, SH Kim, M Choi, YK Bae, C Han, BK Choi, SS Kim, JY Han
Oncogene, 2021-11-06;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
Leukemia, 2021-09-23;0(0):.
Species: Human
Sample Types: Plasma
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Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment
Authors: HP Li, CY Huang, KW Lui, YK Chao, CN Yeh, LY Lee, Y Huang, TL Lin, YC Kuo, MY Huang, YR Lai, YM Yeh, HC Fan, AC Lin, JC Hsieh, KP Chang, CY Lin, HM Wang, YS Chang, CL Hsu
Cancers, 2021-06-12;13(12):.
Species: Human
Sample Types: Plasma
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NRF2 Enables EGFR Signaling in Melanoma Cells
Authors: JKC Kre beta, C Jessen, A Marquardt, A Hufnagel, S Meierjohan
International Journal of Molecular Sciences, 2021-04-07;22(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Enhanced MCP-1 Release in Early Autosomal Dominant Polycystic Kidney Disease
Authors: P Janssens, JP Decuypere, S De Rechter, L Breysem, D Van Giel, J Billen, A Hindryckx, L De Catte, M Baldewijns, KBM Claes, KM Wissing, K Devriendt, B Bammens, I Meyts, VE Torres, R Vennekens, D Mekahli
Kidney international reports, 2021-04-06;6(6):1687-1698.
Species: Human
Sample Types: Urine
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Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators
Authors: S Isozaki, H Konishi, M Fujiya, H Tanaka, Y Murakami, S Kashima, K Ando, N Ueno, K Moriichi, T Okumura
Mediators of Inflammation, 2021-03-29;2021(0):5582943.
Species: Human
Sample Types: Plasma
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In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
International Journal of Molecular Sciences, 2021-03-16;22(6):.
Species: Human
Sample Types: Plasma
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Expression, Purification and Functional Identification of the Modified hEGF Protein
Authors: H Guo, F Hu, K Li, Z Zhao, Z Li, X Xu, X Gou
Protein Expr Purif, 2020-11-14;179(0):105787.
Species: Human
Sample Types: Tissue Homogenates
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Anti-epidermal growth factor vaccine antibodies increase the antitumor activity of kinase inhibitors in ALK and RET rearranged lung cancer cells
Authors: J Codony-Ser, S García-Rom, MÁ Molina-Vil, J Bertran-Al, S Viteri, E d'Hondt, R Rosell
Transl Oncol, 2020-10-23;14(1):100887.
Species: Human
Sample Types: Cell Culture Supernates
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LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells
Authors: S Kim, H Cho, SO Hong, SJ Oh, HJ Lee, E Cho, SR Woo, JS Song, JY Chung, SW Son, SM Yoon, YM Jeon, S Jeon, C Yee, KM Lee, SM Hewitt, JH Kim, KH Song, TW Kim
Autophagy, 2020-08-14;0(0):1-20.
Species: Human
Sample Types: Cell Culture Supernates
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Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
Int J Mol Sci, 2020-06-04;21(11):.
Species: Human
Sample Types: Tissue Homogenates
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Anti-EGFR Therapy Induces EGF Secretion by Cancer-Associated Fibroblasts to Confer Colorectal Cancer Chemoresistance
Authors: CM Garvey, R Lau, A Sanchez, RX Sun, EJ Fong, ME Doche, O Chen, A Jusuf, HJ Lenz, B Larson, SM Mumenthale
Cancers (Basel), 2020-05-28;12(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas
Authors: S Di Marco, S Riccitelli, M Di Paolo, E Campos, M Buzzi, S Bisti, P Versura
Biomolecules, 2020-04-28;10(5):.
Species: Human
Sample Types: Cord Blood Serum
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MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells
Authors: Z Dong, J Zou, J Li, Y Pang, Y Liu, C Deng, F Chen, H Cui
Cancer Med, 2019-11-05;0(0):.
Species: Human
Sample Types: Whole Cells
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CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production
Authors: ASMR Haque, M Moriyama, K Kubota, N Ishiguro, M Sakamoto, A Chinju, K Mochizuki, T Sakamoto, N Kaneko, R Munemura, T Maehara, A Tanaka, JN Hayashida, S Kawano, T Kiyoshima, S Nakamura
Sci Rep, 2019-10-10;9(1):14611.
Species: Human
Sample Types: Cell Culture Supernates
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Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
Cell Transplant, 2019-01-24;0(0):9636897198256.
Species: Human
Sample Types: Cell Culture Supernates
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Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease
Authors: B Satirapoj, R Dispan, P Radinahame, C Kitiyakara
BMC Nephrol, 2018-09-21;19(1):246.
Species: Human
Sample Types: Urine
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1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
PLoS ONE, 2018-09-06;13(9):e0203048.
Species: Human
Sample Types: Cell Culture Supernates
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Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
Stem Cell Res Ther, 2018-05-02;9(1):128.
Species: Human
Sample Types: Cell Culture Supernates
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Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer
Authors: P Xing, H Wang, S Yang, X Han, Y Sun, Y Shi
BMC Immunol., 2018-04-16;19(1):14.
Species: Human
Sample Types: Serum
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Deterioration in saliva quality in patients with Sj�gren's syndrome: impact of decrease in salivary epidermal growth factor on the severity of intraoral manifestations
Authors: N Azuma, Y Katada, H Sano
Inflamm Regen, 2018-04-09;38(0):6.
Species: Human
Sample Types: Saliva
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Comparative analyses of industrial-scale human platelet lysate preparations.
Authors: Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems
Transfusion, 2017-10-08;0(0):1537-2995.
Species: Human
Sample Types: Plasma
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Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
Sci Rep, 2017-08-16;7(1):8484.
Species: Human
Sample Types: Cell Culture Supernates
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Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
PLoS ONE, 2017-08-10;12(8):e0182716.
Species: Human
Sample Types: Tissue Homogenates
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Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
PLoS ONE, 2017-07-26;12(7):e0181214.
Species: Human
Sample Types: Plasma
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The p38 signaling pathway mediates quiescence of glioma stem cells by regulating epidermal growth factor receptor trafficking
Authors: A Soeda, J Lathia, BJ Williams, Q Wu, J Gallagher, A Androutsel, AJ Giles, C Yang, Z Zhuang, MR Gilbert, JN Rich, DM Park
Oncotarget, 2017-05-16;8(20):33316-33328.
Species: Human
Sample Types: Cell Culture Supernates
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Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
J Am Heart Assoc, 2017-05-03;6(5):.
Species: Human
Sample Types: Plasma
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The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
J Transl Med, 2017-05-01;15(1):90.
Species: Human
Sample Types: Cell Lysates
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C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers
Authors: L Shen, P Zhang, S Zhang, L Xie, L Yao, W Lang, J Lian, W Qin, M Zhang, L Ji
Exp Ther Med, 2017-04-05;13(6):3021-3031.
Species: Human
Sample Types: Cell Culture Supernates
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Urine Epidermal Growth Factor, Monocyte Chemoattractant Protein-1 or Their Ratio as Biomarkers for Interstitial Fibrosis and Tubular Atrophy in Primary Glomerulonephritis
Authors: Supanat Worawichaw
Kidney Blood Press. Res, 2016-12-19;41(6):997-1007.
Species: Human
Sample Types: Urine
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Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
Authors: Anne Fischer-Ni
Cytotherapy, 2016-11-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts
Authors: Gorka Orive
Eur. J. Obstet. Gynecol. Reprod. Biol., 2016-09-22;206(0):125-130.
Species: Human
Sample Types: Plasma
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N-Glycosylation of integrin ?5 acts as a switch for EGFR-mediated complex formation of integrin ?5?1 to ?6?4
Sci Rep, 2016-09-19;6(0):33507.
Species: Human
Sample Types: Cell Culture Supernates
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Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
Stem Cell Res Ther, 2016-09-09;7(1):128.
Species: Human
Sample Types: Cell Culture Supernates
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Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
Int J Mol Sci, 2016-08-03;17(8):.
Species: Human
Sample Types: Serum
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Transgenic Soybean Production of Bioactive Human Epidermal Growth Factor (EGF)
Authors: Yonghua He
PLoS ONE, 2016-06-17;11(6):e0157034.
Species: Plant - Arabidopsis
Sample Types: Protein
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Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
Authors: Chou M, Burnouf T, Wang T
PLoS ONE, 2014-06-19;9(6):e99145.
Species: Human
Sample Types: Cell Culture Supernates
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Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype.
Authors: Zhang Z, Dong Z, Lauxen I, Filho M, Nor J
Cancer Res, 2014-03-31;74(10):2869-81.
Species: Human
Sample Types: Cell Culture Supernates
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The evolution of cellular deficiency in GATA2 mutation.
Authors: Dickinson R, Milne P, Jardine L, Zandi S, Swierczek S, McGovern N, Cookson S, Ferozepurwalla Z, Langridge A, Pagan S, Gennery A, Heiskanen-Kosma T, Hamalainen S, Seppanen M, Helbert M, Tholouli E, Gambineri E, Reykdal S, Gottfreethsson M, Thaventhiran J, Morris E, Hirschfield G, Richter A, Jolles S, Bacon C, Hambleton S, Haniffa M, Bryceson Y, Allen C, Prchal J, Dick J, Bigley V, Collin M
Blood, 2013-12-17;123(6):863-74.
Species: Human
Sample Types: Serum
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Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
Species: Human
Sample Types: Serum
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Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
Clin Cancer Res, 2012-07-27;18(22):6219-26.
Species: Human
Sample Types: Cell Culture Supernates
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Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, RuÃÂz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
Clin. Cancer Res., 2012-02-28;18(8):2290-300.
Species: Human
Sample Types: Plasma
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Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
BMC Cancer, 2011-07-05;11(0):286.
Species: Human
Sample Types: Serum
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Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia, 2011-06-10;54(9):2358-67.
Species: Human
Sample Types: Serum
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Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
ALTEX, 2011-01-01;28(4):305-16.
Species: Human
Sample Types: Cell Lysates
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Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
Authors: Lee HS, Myers A, Kim J
Am. J. Respir. Crit. Care Med., 2009-09-17;180(11):1056-67.
Species: Human
Sample Types: Nasal Lavage Fluid
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Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases.
Authors: Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR
Biochem. J., 2009-08-13;422(2):383-92.
Species: Human
Sample Types: Cell Culture Supernates
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Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
Authors: Farrow B, Berger DH, Rowley D
J. Surg. Res., 2009-05-03;156(1):155-60.
Species: Human
Sample Types: Cell Culture Supernates
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A urinary biomarker profile for children with HIV-associated renal diseases.
Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
Kidney Int., 2009-04-08;76(2):207-14.
Species: Human
Sample Types: Urine
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Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer.
Authors: Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP
J. Clin. Oncol., 2009-03-30;27(17):2751-7.
Species: Human
Sample Types: Plasma
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Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer.
Authors: Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
Br. J. Cancer, 2009-01-06;100(2):298-304.
Species: Human
Sample Types: Serum
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Effect of protein kinase Cbeta inhibition on renal hemodynamic function and urinary biomarkers in humans with type 1 diabetes: a pilot study.
Authors: Cherney DZ, Konvalinka A, Zinman B, Diamandis EP, Soosaipillai A, Reich H, Lorraine J, Lai V, Scholey JW, Miller JA
Diabetes Care, 2008-10-22;32(1):91-3.
Species: Human
Sample Types: Urine
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Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
Clin. Cancer Res., 2008-05-01;14(9):2785-2795.
Species: Human
Sample Types: Cell Culture Supernates
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Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine.
Authors: Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T
Clin. Cancer Res., 2008-02-01;14(3):840-6.
Species: Human
Sample Types: Serum
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The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
Fertil. Steril., 2007-11-05;90(1):60-4.
Species: Human
Sample Types: Follicular Fluid
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Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma.
Authors: Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M
Br. J. Cancer, 2007-04-24;96(10):1569-78.
Species: Human
Sample Types: Serum
-
Cytokine profiling in human colostrum and milk by protein array.
Authors: Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, Tuckova L, Tlaskalova-Hogenova H
Clin. Chem., 2007-03-15;53(5):955-62.
Species: Human
Sample Types: Milk
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Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
Int. J. Cancer, 2007-02-01;120(3):686-93.
Species: Human
Sample Types: Cell Culture Supernates
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Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
Cancer Res., 2007-01-15;67(2):555-62.
Species: Human
Sample Types: Cell Culture Supernates
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Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
J. Mol. Endocrinol., 2005-04-01;34(2):353-66.
Species: Human
Sample Types: Cell Culture Supernates
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Salivary epidermal growth factor in oral cavity cancer.
Authors: Balicki R, Grabowska SZ, Citko A
Oral Oncol., 2005-01-01;41(1):48-55.
Species: Human
Sample Types: Saliva
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A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31.
Species: Human
Sample Types: Cell Lysates
-
Alterations in antioxidant power and levels of epidermal growth factor and nitric oxide in saliva of patients with inflammatory bowel diseases.
Authors: Jahanshahi G, Motavasel V, Rezaie A, Hashtroudi AA, Daryani NE, Abdollahi M
Dig. Dis. Sci., 2004-11-01;49(11):1752-7.
Species: Human
Sample Types: Plasma
-
Factors associated with the outcomes of children with unilateral ureteropelvic junction obstruction.
Authors: Chiou YY, Chiu NT, Wang ST, Cheng HL, Tang MJ
J. Urol., 2004-01-01;171(1):397-402; disc.
Species: Human
Sample Types: Urine
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AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
Exp. Neurol., 2002-06-01;175(0):318.
Species: Rat
Sample Types: Cell Culture Supernates
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Saliva analysis in the clinical setting: revisiting an underused diagnostic tool.
Authors: Nagler RM, 2019, Hershkovich O, Lischinsky S, Diamond E, Reznick AZ
1333-1343.e7, 2002-05-01;50(3):214-25.
Species: Human
Sample Types: Saliva
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Epithelial cells in nasal fluids from patients with allergic rhinitis: how do they relate to epidermal growth factor, eosinophils and eosinophil cationic protein?
Authors: Benson M, Uddman R, Cardell LO
Acta Otolaryngol., 2002-03-01;122(2):202-5.
Species: Human
Sample Types: Nasal Lavage Fluid
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The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx.
Authors: Chen CD, Yang JH, Lin KC, Chao KH, Ho HN, Yang YS
Hum. Reprod., 2002-01-01;17(1):128-33.
Species: Human
Sample Types: Hydrosalpinx Fluid
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Diminished epidermal growth factor levels in infants with necrotizing enterocolitis.
Authors: Shin CE, Falcone RA, Stuart L, Erwin CR, Warner BW
J. Pediatr. Surg., 2000-02-01;35(2):173-6; discus.
Species: Human
Sample Types: Serum
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We analyzed urine at a 150-fold dilution and plasma/serum at a 10-fold dilution. The standard curve had an excellent 4PL fit. We continue to use this assay routinely.